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Synthesis, Reconstitution and EPR Spectroscopy of Artificial Quinone Electron Acceptors in Reaction Centres from Purple Bacteria

  • Ben-Li Liu
  • Lihua Yang
  • Monika R. Fischer
  • Arnold J. Hoff

Abstract

Isolated RC’s from both Rb. sphaeroides R26 and Rps. viridis can be depleted of the natural quinones and then reconstituted with artificial quinones of different thermodynamic and stereochemical properties. Assaying these RC’s with optical flash and ESR spectroscopy allows to gain insight in the thermodynamics of the primary reactions in photosynthesis, and in the structural requirements of the binding site of the quinone acceptors.

Keywords

Difference Spectrum Structural Requirement Primary Reaction Purple Bacterium Quinone Acceptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Okamura, M. Y. and Feher, G. (1974) Biochem. 13 1394–1403CrossRefGoogle Scholar
  2. 2.
    Okamura, M. Y. et al (1975) Proc. Nat. Acad. Sci. USA 72 9 3491–3495PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Ben-Li Liu
    • 1
  • Lihua Yang
    • 1
  • Monika R. Fischer
    • 1
  • Arnold J. Hoff
    • 1
  1. 1.Dept. of Biophysics, Huygens LaboratoryLeiden State UniversityLeidenThe Netherlands

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