Synthesis, Reconstitution and EPR Spectroscopy of Artificial Quinone Electron Acceptors in Reaction Centres from Purple Bacteria
Isolated RC’s from both Rb. sphaeroides R26 and Rps. viridis can be depleted of the natural quinones and then reconstituted with artificial quinones of different thermodynamic and stereochemical properties. Assaying these RC’s with optical flash and ESR spectroscopy allows to gain insight in the thermodynamics of the primary reactions in photosynthesis, and in the structural requirements of the binding site of the quinone acceptors.
KeywordsDifference Spectrum Structural Requirement Primary Reaction Purple Bacterium Quinone Acceptor
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