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The Transient Photosystem I EPR Spectra Correlated Spin Pair Concept and Structural Conclusions

  • Dietmar Stehlik
  • Christian H. Bock
  • Arthur J. Van Der Est

Abstract

For many years it has been known that primary charge separation in photosynthetic reaction centers is associated with spin polarized EPR-signals. Such signals can be detected directly in preparations containing PS I whereas their observation in bacterial reaction centers or PS II requires removal or decoupling of the non-heme iron, which acts as a strong relaxation center. However, their usefullness has been hampered for some time by a lack of understanding of their origin. With the present knowledge of the molecular arrangement of the chromophores in the bacterial reaction center it is obvious that transient EPR-de-tection occurs in coupled spin pairs. With this concept /1–5/ observed transient EPR spectra can be understood satisfactorily /6/. Here, we want to address some of the questions raised in the context of this interpretation and present confirming evidence for the major conclusions reached previously /6/.

Keywords

Microwave Field Photosynthetic Reaction Center Spin Pair Primary Charge Separation Bacterial Reaction Center 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1a.
    Thurnauer, M. C. and Norris, J. R. (1980) Chem. Phys. Lett. 76, 557–561;CrossRefGoogle Scholar
  2. 1b.
    Thurnauer, M. C. and Meisel, D. J. (1983) J. Am. Chem. Soc. 105, 3729–3731CrossRefGoogle Scholar
  3. 2.
    Closs, G. L., Forbes, M. D. E. and Norris, J. R. (1987) J. Phys. Chem. 91, 3592–3599CrossRefGoogle Scholar
  4. 3.
    Closs, G. L. and Forbes, M. D. E. (1987) J. Am. Chem. Soc. 109, 6185–6187CrossRefGoogle Scholar
  5. 4.
    Buckley, C. D., Hunter, D. A. and Mc Lauchlan, K. A. (1987) Chem. Phys. Lett. 135, 307–312CrossRefGoogle Scholar
  6. 5.
    Hore, P. J., Hunter, D. A., McKie, C. D. and Hoff, A. J. (1987) Chem. Phys. Lett. 137, 495–500CrossRefGoogle Scholar
  7. 6.
    Stehlik, D., Bock, C. H. and Petersen, J. (1989) J. Phys. Chem. 93, 1612–1619CrossRefGoogle Scholar
  8. 7.
    Bock, C. H., van der Est, A. J., Brettel, K. and Stehlik, D. (1989) FEBS Lett. 247, 91–96CrossRefGoogle Scholar
  9. 8.
    Hoff, A. J., (1984) Quart. Rev. Biophys. 7, 153–282CrossRefGoogle Scholar
  10. 9.
    Bock, C. H., Stehlik, D., Thurnauer, M. C. (1988), Isr. J. Chem. 28, 177–182Google Scholar
  11. 10.
    Stehlik, D., Bock, C. H., Thurnauer, M. C. (1989) in Advanced EPR in Biology and Biochemistry (Hoff, A. J. ed.) Chap. 12, Elsevier, AmsterdamGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Dietmar Stehlik
    • 1
  • Christian H. Bock
    • 1
  • Arthur J. Van Der Est
    • 1
  1. 1.FB PhysikFU BerlinBerlin 33Germany

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