Advertisement

Reconstitution of Photochemical Activity in Rhodobacter capsulatus Reaction Centers Containing Mutations at Tryptophan M-250 in the Primary Quinone Binding Site

  • W. J. Coleman
  • E. J. Bylina
  • D. C. Youvan

Abstract

Tryptophan M-250 (TrpM250) in the reaction center (RC) of the purple non-sulfur bacteria occupies a key position between the photoactive pheophytin (HA) and the primary quinone (QA), according to the X-ray crystal structures obtained for Rps. viridis and Rb. sphaeroides (refs. 1,2; Fig. 1). Seven directed mutations have been introduced at this position in a related organism, Rb. capsulatus, in order to determine their effects on quinone binding and electron transfer (for a review, see ref.3). The results of these substitutions indicate that although TrpM250 is important for high-affinity quinone binding, primary photochemistry can be reconstituted in all of the genetically modified RCs by addition of exogenous quinone.

Keywords

Photochemical Activity Primary Photochemistry Photosynthetic Growth Quinone Binding Indole Side Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Michel, H., Epp, O. and J. Deisenhofer (1986) EMBO J. 5, 2445–2451.PubMedGoogle Scholar
  2. 2.
    Allen, J.P., Feher, G., Yeates, T.O., Komiya, H. and D.C. Rees (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 8487–8491.PubMedCrossRefGoogle Scholar
  3. 3.
    Coleman, W.J. and D.C. Youvan (1990) Ann. Rev. Biophys. Biophys. Chem, submitted.Google Scholar
  4. 4.
    Bylina, E.J., Jovine, R.V.M. and D.C. Youvan (1989) Bio/technology 7, 69–74.CrossRefGoogle Scholar
  5. 5.
    Prince, R. C. and D.C. Youvan (1987) Biochim. Biophys. Acta 890, 286–291.CrossRefGoogle Scholar
  6. 6.
    Okamura, M.Y., Isaacson, R.A. and G. Feher (1975) Proc. Natl. Acad. Sci. U.S.A. 72, 3491–3495.PubMedCrossRefGoogle Scholar
  7. 7.
    Gunner, M.R., Braun, B.S., Bruce, J.M. and P.L Dutton (1985) In: Antennas and reaction centers of photosynthetic bacteria (Michel-Beyerle, M.E., ed) Springer-Verlag, New York, pp. 298–305.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • W. J. Coleman
    • 1
  • E. J. Bylina
    • 1
  • D. C. Youvan
    • 1
  1. 1.Department of ChemistryMassachusetts Institute of TechnologyCambridgeUSA

Personalised recommendations