Spectroscopic and Orientational Properties of Chlorophylls a and b in Lipid Membranes
The efficiency of energy transfer in photosynthesis depends on the relative orientations of the emission and absorption transition moments respectively of the donor and acceptor chlorophyll molecules. This is determined by the mutual orientation of the chromophores within the structure of the photosynthetic membranes. In order to gain a better insight into the molecular mechanism of energy transfer, it is necessary to characterize the directions of the transition moments in the frame of the chromophores. This can be achieved by combining the results of angle-resolved linear dichroism and fluorescence depolarisation measurements on macroscopically oriented lipid membranes with the results of emission-resolved fluorescence anisotropy data from isotropic systems (vesicles) (1).
KeywordsFluorescence Anisotropy Transition Moment Linear Dichroism Bilayer Plane Fluorescence Anisotropy Measurement
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