Spectroscopic and Orientational Properties of Chlorophylls a and b in Lipid Membranes

  • Marnix van Gurp
  • Gijs Van Ginkel
  • Yehudi K. Levine

Abstract

The efficiency of energy transfer in photosynthesis depends on the relative orientations of the emission and absorption transition moments respectively of the donor and acceptor chlorophyll molecules. This is determined by the mutual orientation of the chromophores within the structure of the photosynthetic membranes. In order to gain a better insight into the molecular mechanism of energy transfer, it is necessary to characterize the directions of the transition moments in the frame of the chromophores. This can be achieved by combining the results of angle-resolved linear dichroism and fluorescence depolarisation measurements on macroscopically oriented lipid membranes with the results of emission-resolved fluorescence anisotropy data from isotropic systems (vesicles) (1).

Keywords

Fluorescence Anisotropy Transition Moment Linear Dichroism Bilayer Plane Fluorescence Anisotropy Measurement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Van Gurp, M., van Ginkel, G. and Levine, Y.K. (1988), J. Theor. Biol. 333–349.Google Scholar
  2. 2.
    Van Gurp, M. and Levine, Y.K. (1989) J. Chem Phys. 90, 4095–4102.CrossRefGoogle Scholar
  3. 3.
    Van Gurp, M., van Ginkel, G. and Levine Y.K. (1989) Biochim Biophys. Acta 973, 405–413.CrossRefGoogle Scholar
  4. 4.
    Levine, R.D. and Tribus, M. (1979) The Maximum Entropy Formalism, MIT Press, Cambridge.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Marnix van Gurp
    • 1
  • Gijs Van Ginkel
    • 1
  • Yehudi K. Levine
    • 1
  1. 1.Department of Molecular Biophysics, Buys Ballot LaboratoryUniversity of UtrechtUtrechtThe Netherlands

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