Restoration of an Oligomeric Form of the Light-Harvesting Antenna CP II and of a Fluorescence State II-State I Transition by Δ3-Trans-Hexadecenoic Acid-Containing Phosphatidylglycerol, in a Mutant of Chlamydomonas
Phosphatidylglycerol (PG) containing Δ3-trans-hexadecenoic acid (C16:1-trans) is a phospholipid characteristic of the photosynthetic membranes. Several works concerning higher plants (1–4) or the unicellular green alga Chlamydomonas reinhardtii (5,6) have suggested that C16:1-trans could be involved in the formation and the stability of an oligomeric form of the main light-harvesting Ch1 a + b antenna (LHCP or CP II) and, consequently, in the efficiency of the light energy capture and transfer. However, other observations have not agreed with such a suggestion (7,8). We report here a restoration experiment using two mutants of C. reinhartdii; mf 2, a PS II-lacking but low-fluorescent mutant which is unable to synthesize C16;1-trans, does not show any oligomeric form of CP II and shows anomalies affecting the regulation of the excitation energy distribution; Fl 39, a classical PS II-lacking high-fluorescent mutant, used as a control.
KeywordsFluorescence Emission Spectrum Oligomeric Form Photosynthetic Membrane Unicellular Green Alga Alga Suspension
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