Proteolytic Digestion of the N-Terminus of the Extrinsic 33-kDa Protein of the Photosystem II Complex
Three extrinsic proteins, located on the luminal side of PSII, participate in oxygen evolution« Molecular masses for these proteins have been designated as 33, 23 and 18 kDa from SDS-PAGE (for a review see ). Partial degredation of the N-terminal portion of the 18- and 23-kDa proteins suggested that this region contains a domain for binding to the PSII complex [2,3]. In the present work we subjected the 33-kDa protein to limited proteolysis using chymotrypsin and Staphylococcus aureus (Strain V8) protease. These proteolytic enzymes eliminated 16 and 18 amino acid residues, respectively, from the N-terminal region of the protein. The remaining large fragments could not rebind to PSII.
KeywordsOxygen Evolution Circular Dichroism Spectrum PSII Complex Extrinsic Protein PSII Membrane
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