Reversible Inhibition of DCMU on the Oxidizing Side of Photosystem II

  • Hiroshi Inoué
  • Hiroyuki Kamachi
  • Tohoru Kitamura
  • Noriaki Tamura


Two sites of inhibition in photosystem II (PS II) have been shown for 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). The primary inhibition site which has been well characterized is localized at Q, which is harbored by D1 protein (1). The second inhibition site of DCMU has been reported to exist on the oxidizing side of PS II in chloroplasts (2, 3) or Triton-prepared PS II membranes (4,5). However, it remains to be solved which species on the oxidizing side of PSII react(s) with EXMJ in detail, since it is very difficult to distinguish between two inhibitions by the herbicide. Interestingly, results have been accumulated that D1 protein possesses functional components of the oxidizing side of PS II such as the Mn ligation site (6), Z and D (7), and P680 (8), in addition to those on the PS II reducing side. In this report, with Tris-treated PS II membranes (Tris-TMF2), we confirmed the dual inhibitions by DCMU of PS II, and found that DCMU modifies the high-affinity Mn-binding site, resulting in the inhibition of photoligation of Mn2+ into the apo-Mn complex.


Chlorophyll Fluorescence Oxygen Evolution Inhibition Site PSII Membrane Diphenyl Carbazide 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Hiroshi Inoué
    • 1
  • Hiroyuki Kamachi
    • 1
  • Tohoru Kitamura
    • 1
  • Noriaki Tamura
    • 1
  1. 1.Department of Biology, Faculty of ScienceToyama UniversityToyama 930Japan

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