Oxygen Evolution of Lyophilized Photosystem II Membranes

  • Kunio Kawamoto
  • Kozi Asada


Lyophilized photosystem (PS) II membranes showed 70% of the activity of oxygen evolution of the control PS II membranes. The yield of the flash-induced oxygen evolution of the lyophilized PS II membranes peaked at the sixth flash in aqueous medium, indicating that the water-oxidizing enzyme was apparently reduced to “S−2 state” by lyophilization. When the lyophilized PS II membranes were adapted in the dark after preillumination in aqueous medium, the oxygen evolution oscillated as the control showing the maximum at the third flash. On the other hand, when preilluminated under dry conditions, the oxygen evolution was maximal at the sixth flash. These results indicate that the water bound to the water-oxidizing enzyme is depleted by lyophilization, and two photons and the strongly bound water are required for recovering the S0 from S−2 states.


Oxygen Evolution Photosynthetic Oxygen Evolution White Continuous Light Quadrupole Mass Analyzer Flash Number 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Kunio Kawamoto
    • 1
  • Kozi Asada
    • 1
  1. 1.The Research Institute for Food ScienceKyoto UniversityUji, Kyoto 611Japan

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