Characterization of Monoclonal Antibodies Which Recognize the 33, 24, and 17 kDa Extrinsic Proteins of Photosystem II

  • Terry M. Bricker
  • Laurie K. Frankel


At least seven proteins appear to be required for photosynthetic oxygen evolution. These proteins, the 47 kDa apoprotein of CPa1, the 45 kDa apoprotein of CPa2, the D1 and D2 proteins, the extrinsic 33 kDa manganese-stabilizing protein, and the 9 and 4.5 kDa subunits of cytochrome b559, form an isolatable oxygen-evolving core preparation which exhibits high rates of water oxidation [1], Additionally, in vivo, at least two other proteins with the molecular masses of 24 and 17 kDa are associated with the oxygen-evolving complex (2).


ELISA Plate Water Oxidation Extrinsic Protein Photosynthetic Oxygen Evolution Resuspension Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Ghanotakis, D.F., Demetriou, D.M. and Yocum, C.F. (1987) Biochim. Bio phys. Acta 891,15–21.CrossRefGoogle Scholar
  2. 2.
    Kuwabara, T. and Murata, N. (1983) Plant Cell Physiol. 24,741–747.Google Scholar
  3. 3.
    Miyao, M. and Murata, N. (1984) FEBS Lett. 170,350–354.CrossRefGoogle Scholar
  4. 4.
    Wales, R., Newman, B.J., Pappin, D. and Gray, J.C. Plant Mol. Biol. 12,439–451.Google Scholar
  5. 5.
    Frankel, L.K. and Terry M. Bricker (1987) Arch. Biochem. Biophys. 256, 295–301.PubMedCrossRefGoogle Scholar
  6. 6.
    Yamamoto, Y., Hermodson, M.A. and Krogmann, D.W. (1986) FEBS Lett. 195, 155–158.CrossRefGoogle Scholar
  7. 7.
    Bricker, T.M., Odom, W.R. and Queirolo, C.B. (1988) FEBS Lett. 231,111–117.CrossRefGoogle Scholar
  8. 8.
    Delepelaire, P. and Chua, N.-H (1979) Proc. Natl. Acad. Sci. (USA) 76,111–115.CrossRefGoogle Scholar
  9. 9.
    Towbin, H., Staehelin, T. and Gordon, J. (1979) Proc. Natl. Acad. Sci. (USA) 76,4350–4354.CrossRefGoogle Scholar
  10. 10.
    Ghanotokis, D. and Yocum, C.F. (1985) Photosyn. Res. 7,97–114.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Terry M. Bricker
    • 1
    • 2
  • Laurie K. Frankel
    • 1
  1. 1.Department of BotanyLouisiana State UniversityBaton RougeUSA
  2. 2.Department of BiochemistryLouisiana State UniversityBaton RougeUSA

Personalised recommendations