Reversibility of Variable Fluorescence at 77 K up to 100% Indicating the Redox States of QA and QB in Leaves

  • Beatrix Schwarz
  • Reto J. Strasser


Leaves, needles, algae and chloroplasts can be frozen to 77K. In this stage the fluorescence kinetics can be measured one or several times according to the dark time given between two excitations. The dynamic behaviour appears to be hyperbolic which confirms the hypothesis of Strasser et al.(1, 2, 3). Reversibility of variable fluorescence has been observed earlier (4, 5, 6) and interpreted in a different manner. The following experiments can be considered as a powerful tool to describe the energetics of primary reactions of photosynthesis in a new way. The technique gives acces to the description of Q(A) and Q(B) in situ of e. g. whole leaves.


Hyperbolic Function Fluorescence Induction Variable Fluorescence Plot Versus Fluorescence Kinetic 
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  1. 1.
    Strasser, R.J. (1978) in Chloroplast Development (Akoyunoglou, G., ed.), pp. 513–524, Elsevier, North-HollandGoogle Scholar
  2. 2.
    Strasser, R.J. (1981) in Photosynthesis III (Akoyunoglou, G., ed.) pp. 727–737, Balaban International Science service, PhiladelphiaGoogle Scholar
  3. 3.
    Strasser, R.J. and Greppin, H. (1981) in Photosynthesis III (Akoyounoglou, G., ed.), pp. 717–726, Balaban Int., PhiladelphiaGoogle Scholar
  4. 4.
    Joliot, P. et al (1973), Biochim. Biophys. Acta 305, 317–328PubMedCrossRefGoogle Scholar
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    Strasser, R.J. and Graf, J.A. (1987), 2nd int. Symp. Air Pollut. and Plant.Metab., GSF-Bericht 9, 19–26 München FRGGoogle Scholar
  6. 6.
    Strasser, R.J. (1989), these proceedingsGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Beatrix Schwarz
    • 1
  • Reto J. Strasser
    • 1
  1. 1.Bioenergetics LaboratoryUniversity of GenevaLullier - GenevaSwitzerland

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