Effect of Tetranitromethane on Photosystem II Membranes

  • Satoshi Sano
  • Masaaki Takahashi
  • Kozi Asada


Photoiodination of D-1 and D-2 proteins were suppressed in tetranitromethane (TNM)-treated photosystera (PS) II membranes, indicating that TNM treatment of PS II membranes nitrated tyrosine (Tyr) residues including Tyr-161 (Yz) of D-1 protein and Tyr-160 (YD) of D-2 protein. EPR spectra at room temperature, which were identified to be signals IIf and IIs, also indicated the nitration of Yz and YD. By TNM treatment (TNM/Chl ratio was 1.0 (mol/mol) or higher), the oxygen evolution was half inhibited, but the DPC-supported DCIP reduction was not affected. TNM completely quenched the long-term delayed luminescence of thylakoids and PSII membranes. Thus, YD of PSII reaction center might be on a mediator for the charge recombination between S2 and the secondary electron acceptors.


Oxygen Evolution PSII Reaction Center Hamamatsu Photonic Primary Electron Donor PSII Membrane 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Satoshi Sano
    • 1
  • Masaaki Takahashi
    • 2
  • Kozi Asada
    • 1
  1. 1.The Research Institute for Food ScienceKyoto Univ.Uji, Kyoto 611Japan
  2. 2.Dept. of BiologyKonan Univ.Kobe 658Japan

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