Advertisement

Kinetic Absorption Spectroscopy Provides Evidence for the Function of Z in (D1,D2) Photosystem-II Reaction Centers

  • P. Mathis
  • K. Satoh
  • Ö. Hansson

Abstract

In Photosystem-II (PS-II), light excitation induces the oxidation of the primary donor P-680, and the reduction of the primary acceptor, a pheophytin I. After a short flash, QA is reduced by l in less than one nanosecond and the reduction of P-680+ by the secondary donor Z takes times which vary from the 50 ns domain, under physiological conditions, to the 5–50 µs domain when oxygen evolution is inhibited. A PS-II reaction center complex has been recently isolated (1). It contains two polypeptides named D1 and D2 which hold the primary photochemical partners P-680 and I, and cytochrome b559, but QA is absent. According to recent results obtained by site-directed mutagenesis (2,3) and by specific iodination (4), Z is one of the tyrosines (Tyr-161) of the polypeptide D1. Thus Z should be present in purified (D1,D2 PS-II complexes. There is, however, no firm functional evidence for that presence. EPR measurements following illumination at low temperature, led to propose that a donor to P-680+ was operating, but its chemical identification with Z could not be obtained (5,6).

Keywords

Fast Phase Reaction Center Complex Microsecond Range Short Flash Reaction Center Core 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. (1).
    Nanba, O. and Satoh, K. (1987) Proc.Natl.Acad Sci. USA 84, 109–112.PubMedCrossRefGoogle Scholar
  2. (2).
    Debus, R.J., Barry, B.A., Sithole, I., Babcock, G.T. and McIntosh, L (1988) Biochemistry 27, 9071–9074.PubMedCrossRefGoogle Scholar
  3. (3).
    Vermaas, W., Rutherford, A.W. and Hansson, Ö. (1988) Proc. Nat. Acad. Sci. 85, 8477–8481CrossRefGoogle Scholar
  4. (4).
    Takahashi, Y. and Satoh, K. (1989) Biochim.Biophys.Acta 973, 138–146.CrossRefGoogle Scholar
  5. (5).
    Demetriou, C., Lockett, D.J. and Nugent, J.H.A. (1988) Biochem.J. 252, 921–924.PubMedGoogle Scholar
  6. (6).
    Frank, H.A., Hansson, Ö. and Mathis, P. (1989) Photosynth.Res. 20, 279–289.Google Scholar
  7. (7).
    Takahashi, Y., Hansson, Ö., Mathis, P. and Satoh, K. (1987) Biochim.Biophys.Acta 893, 49–59.CrossRefGoogle Scholar
  8. (8).
    Chapman, D.J., Gounaris, K. and Barber, J. (1988) Biochim.Biophys.Acta 933, 423-431.CrossRefGoogle Scholar
  9. (9).
    Gounaris, K., Chapman, D.J. and Barber, J. (1988) FEBS Lett. 240, 143–147.CrossRefGoogle Scholar
  10. (10).
    Van Best, J.A. and Mathis, P. (1978) Biochim.Biophys.Acta 503, 178–185.PubMedCrossRefGoogle Scholar
  11. (11).
    Conjeaud, H. and Mathis, P. (1980) Biochim.Biophys.Acta 590, 353–359.PubMedCrossRefGoogle Scholar
  12. (12).
    Mathis, P., Satoh, K., and Hansson, O. (1989) FEBS Lett., in press.Google Scholar
  13. (13).
    Satoh, K. and Nakane, H., these proceedingsGoogle Scholar
  14. (14).
    Ikegami, I., Sétif, P. and Mathis, P. (1987) Biochim. Biophys. Acta. 894, 414–422CrossRefGoogle Scholar
  15. (15).
    Telfer, A. and Barber, J. (1989) FEBS Lett. 246, 223–228.CrossRefGoogle Scholar
  16. (16).
    Reinman, S., Mathis, P., Conjeaud, H. and Stewart, A. (1981) Biochim.Biophys.Acta 635, 429–433.PubMedCrossRefGoogle Scholar
  17. (17).
    Boska, M., Sauer, K., Buttner, W. and Babcock, G.T. (1983) Biochim.Biophys.Acta 722, 327–330.CrossRefGoogle Scholar
  18. (18).
    Conjeaud, H., Mathis, P. and Paillotin, G. (1979) Biochim.Biophys.Acta 546, 280–291.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • P. Mathis
    • 1
  • K. Satoh
    • 2
  • Ö. Hansson
    • 3
  1. 1.Département de Biologie, SBPhCEN SACLAYGif-sur-Yvette CedexFrance
  2. 2.Biology DepartmentOkayama UniversityOkayama 700Japan
  3. 3.Department of Biochemistry and BiophysicsUniversity of Göteborg and Chalmers Institute of TechnologyGöteborgSweden

Personalised recommendations