Collagenase synthesis by odontoblast-like cells (RP cells) — preparation of monoclonal antibodies against RP cell collagenase and characterization of the enzyme

  • S. Sakamoto
  • Y. Bolender
  • M. Sakamoto


Rabbit odontoblast-like cells of an established cell line (RP) were shown to secrete substantial amounts of latent collagenase in both serum-supplemented and serum-free cultures. Purified RP cell collagenase revealed three bands of molecular weight 57 kD, 52.5 kD and 45 kD in SDS-polyacrylamide gel electrophoresis. Hybridomas were prepared according to a standard procedure and four monoclonal antibodies (2A6, F9, 2A2 and 2E2) against purified RP cell collagenase were consequently isolated and characterized by various criteria. Western blot analysis of secreted RP cell collagenase with these monoclonal antibodies indicated that the 57 kD, 52.5 kD and 45 kD proteins were all RP cell collagenase species sharing the same epitopes. The possible role of collagenase synthesized by odontoblast-like cells in the degradation of collagen of pathological dental pulp tissue was discussed. Potential applications for this RP cell culture model and the isolated monoclonal antibodies were reviewed.


Dental Pulp Collagenase Activity Dental Pulp Tissue Collagenase Inhibitor Monkey Kidney Epithelial Cell 
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© Chapman and Hall 1990

Authors and Affiliations

  • S. Sakamoto
  • Y. Bolender
  • M. Sakamoto

There are no affiliations available

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