Isozyme and Endosperm Protein Markers in the Determination of Chromosomal Constitution in X Triticosecale Wittmack

  • Pilar Rubio
  • Consuelo Soler
  • Angeles Bernardo
  • Nicolás Jouve
Part of the Developments in Plant Breeding book series (DIPB, volume 5)


The objective of this work is the evaluation of ability of a series of biochemical markers to verify chromosome composition of chromosome segregant materials or secondary lines obtained by crossing triticale and wheat. From all sytems essayed, isozymes of Glutamate Oxalacetate Transaminase, Malate Dehydrogenase and endosperm prolamins studied by SDS-PAGE, were the most effective. GOT, A-PAGE and 2D-PAGE offer the possibility to detect 6D(6A) and other chromosome substitutions.


Hexaploid Wheat Malate Dehydrogenase Chromosome Substitution Glutamate Oxalacetate Transaminase Endosperm Protein 
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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Pilar Rubio
    • 1
  • Consuelo Soler
    • 2
  • Angeles Bernardo
    • 1
  • Nicolás Jouve
    • 1
  1. 1.Department of Cell Biology and GeneticsUniversity of Alcala de HenaresMadridSpain
  2. 2.Department of Plant BreedingC.I.T., I.N.I.A.Alcala de Henares, MadridSpain

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