Isozyme and Endosperm Protein Markers in the Determination of Chromosomal Constitution in X Triticosecale Wittmack

  • Pilar Rubio
  • Consuelo Soler
  • Angeles Bernardo
  • Nicolás Jouve
Part of the Developments in Plant Breeding book series (DIPB, volume 5)

Abstract

The objective of this work is the evaluation of ability of a series of biochemical markers to verify chromosome composition of chromosome segregant materials or secondary lines obtained by crossing triticale and wheat. From all sytems essayed, isozymes of Glutamate Oxalacetate Transaminase, Malate Dehydrogenase and endosperm prolamins studied by SDS-PAGE, were the most effective. GOT, A-PAGE and 2D-PAGE offer the possibility to detect 6D(6A) and other chromosome substitutions.

Keywords

Hexaploid Wheat Malate Dehydrogenase Chromosome Substitution Glutamate Oxalacetate Transaminase Endosperm Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kahler AL, Hallauer AR, Gardner CO. Allozyme polymorphisms within and among open-pollinated and adapted exotic populations of maize. Theor Appl Genet 1986; 72: 592–601.CrossRefGoogle Scholar
  2. 2.
    Marchylo BA. Identification of Canadian and American wheat cultivars by SDS gradient PAGE analysis of gliadin and glutenin subunits. Can J Plant Sci 1987; 67: 945–952.CrossRefGoogle Scholar
  3. 3.
    McIntyre CL.. Variation at isozyme loci in Triticeae. Pl Syst Evol 1988; 160: 123–142.CrossRefGoogle Scholar
  4. 4.
    Bernardo A, Jouve N, Soler C. Discriminaci6n de variedades de cebada (Hordeum vulgare L.) mediante electroforesis. 2. Andlisis de isoenzimas Investigaciôn agraria. Prod Prot Veget 1990; 5 (1): 25–52.Google Scholar
  5. 5.
    Poulik HD. Starch gel eletrophoresis in a discontinuous system of buffers. Nature (London) 1957; 180: 47.CrossRefGoogle Scholar
  6. 6.
    Hart GE. Glutamate oxaloacetate transaminase isozymes of Triticum: evidence for multiple systems of triplicat estructural genes in hexaploid wheat. In: “Isozymes: III Developmental Biology” ( Markert, C. Ed.) New York: Academic Press, 1975: 637–657.Google Scholar
  7. 7.
    Salinas J, Benito C. Chromosomal location of malate dehydrogenase structural genes in rye (Secale cereale L.). Z. Pflanzenzchtg 1985; 94: 208–217.Google Scholar
  8. 8.
    Payne PI, Corfield KG, Holt LM, Blackman JA. Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and breadmaking quality in progenie of six crosses of bread wheat. J Sci Food Agric 1981; 32: 5160.CrossRefGoogle Scholar
  9. 9.
    Lafiandra D, Kasarda D. One-and two-dimensional (two-pH) polyacrilamide gel electrophoresis in a single gel separation of wheat ptoteins. Cereal Chem 1985; 62: 314–319.Google Scholar
  10. 10.
    Tercero JA, Bernardo A, Jouve N. Encoding genes for endosperm proteins in Hordeum chilense. Theor Appl Genet 1990; 81: 127–132.Google Scholar
  11. 11.
    Rubio P, Jouve N, Soler C, Bernardo A. Genetic variation for isozymes and proteins in lines of the synthetic amphiploid x Triticosecale Wittmack. Genetics & Breeding 1994;(in press).Google Scholar
  12. 12.
    Tang KS, Hart GE. Use of isozymes as chromosome markers in wheatÄrye addition lines and in triticale. Genet Res Camb 1975; 26: 187–201.CrossRefGoogle Scholar
  13. 13.
    Scandalios JG Genes, isozymes and evolution. Isozymes IV: Genetics and evolution. San Francisco: Academic press, Inc, 1975.Google Scholar
  14. 14.
    Hart GE, Langston Pi. Chromosome location and evolution of isozyme structural genes in hexaploid wheat. Heredity 1977; 39: 263–277.CrossRefGoogle Scholar
  15. 15.
    Hart GE, Tuleen NA. Introduction and characterization of alien genetic material. Isozymes in Plant Genetics and Breeding, Part A. Amsterdam, Elsevier Science Publishers, 1983:: 339–362.Google Scholar
  16. 16.
    Diaz F, Jouve N. Structure of isozymes of the AAt-2 and AAT-3 systems of Aspartate Amino transaminase in Triticineae. Euphytica 91986; 35: 129–135.CrossRefGoogle Scholar
  17. 17.
    Hart GE. Biochemical loci of hexaploid wheat (Triticum aestivum, 2n=42, genomes AABBDD). Genetics Maps 1984 Vol 3: 485–490.Google Scholar
  18. 18.
    Bergman CW, Salinas J. Isozyme variants of esterase and malate dehydrogenase among wheat aneuploids. Agron Abstr 1972; 23.Google Scholar
  19. 19.
    Benito C, Salinas J. The chromosomal location of malate dehydrogenase isozyme in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 1983; 64: 255–258.CrossRefGoogle Scholar
  20. 20.
    Diaz F, Fernandez JA, Jouve N. Structure and chromosomal location of malate dehydrogenase (Zone 2) isozymes in common and durum wheats. Euphytica 1986; 35: 509–513.CrossRefGoogle Scholar
  21. 21.
    Sozinov A. 1984. Blocks of cereal storage proteins as genetic markers. Proc 2nd Int Workshop on Gluten Proteins, Wageningen: 121–127.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Pilar Rubio
    • 1
  • Consuelo Soler
    • 2
  • Angeles Bernardo
    • 1
  • Nicolás Jouve
    • 1
  1. 1.Department of Cell Biology and GeneticsUniversity of Alcala de HenaresMadridSpain
  2. 2.Department of Plant BreedingC.I.T., I.N.I.A.Alcala de Henares, MadridSpain

Personalised recommendations