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Callus Formation and the Culturability of Inflorescence Primordia of Mutant Triticales

  • Gallia Butnaru
Part of the Developments in Plant Breeding book series (DIPB, volume 5)

Abstract

For a theoretical and practical reason the in vitro culture response of different Triticale genotyps “wild” and mutants was followed. The callusogenesis and culturability of spike explants were decisively influenced by the organogenetic stage.

The best growth medium was 66, MS supplemented with 2.4-D (1 mg/ 1) and Magnetic Particles (MP θ = 0.15 x 10-3 g/cm3) pH 5.8. Callusogenesis ability was positive on mutant genotypes (T. Tim 501-17 - an average 45.2%). The variability was higher at the “wild” regenerants. The FMR1 showed dwarfness (~9.7 cm; 40.4%) with small but normal plant characters. There were nuclear and plastone mutations also (tubular leaf, variegation and lethal chlorophyll deficiency).

The plant characters of FMR2, were more sharply differentiaded. The caulogenesis was strongly connected with the calli size (r = + 0.878).

Keywords

Plant Regeneration Callus Induction Magnetic Fluid Immature Embryo Mutant Genotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Demarly Y., Biotechnologies du clonage des genotypes promotion de l’individu d’elite. In Demarly, Amelioration des plantes et biotechnologies. Ed.John Libbey. Paris, 1989: 45.Google Scholar
  2. 2.
    Babeli P., Karp A., Kaltiskes P.J., Plant regeneration and somaclonal variation from cultured immature embryos of sister lines of rye and triticale differing in their content of heterochromatin. Theor. Appl.Genet. 1988; 75:929.Google Scholar
  3. 3.
    Eapen S., Rao PS., Callus induction and plant regeneration from immature embryos of rye and triticale. Plant. Cell. Tissue Organ Culture. 1982; 1: 221.CrossRefGoogle Scholar
  4. 4.
    Murashige T., Skoog F., A revised medium for a rapid growth and bioassasy with tabacco tissue cultures.Physiol. Plant. 15, 1962:, 473.CrossRefGoogle Scholar
  5. 5.
    Gyulai G., Janovszky J., Kiss E., Lelik L., Csillag A., Heszky LE.,Callus initiation and plant regeneration from inflorescence primordia of intergeneric hybrid Agropyron repens (L) Beauv x Bromus inermis Leyss.CV. nanus on a modified nutritive medium. Plant Cell Reports 1992; 11; 266.CrossRefGoogle Scholar
  6. 6.
    Balan M., Cachita-Cosma D., Grusea A., Callus production and plant regeneration from immature inflorescences of some forage grasses. Lucrarile celui de al V- lea Simpozion national de culturi de celule si tesuturi vegetale. 1993; 303.Google Scholar
  7. 7.
    Butnaru G., Corneanu M., Somatic embryogenesis and plant regeneration in tissue culture in medium with magnetic liquids. Sixth Int.Conf. Magnetic Fluids. Paris, 1992; 478.Google Scholar
  8. 8.
    Butnaru G., Rasa F., Badea M., Micropropagation and mutation in Triticale. In Vitro Plant Cultures, present and perspective; 1989: 69.Google Scholar
  9. 9.
    Butnaru G., Terteac D., Bujoreanu V., Nuclei ultrastructure under MLs influence. Seventh Int. Congr. Genet. Birmingham, 1993: 140.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Gallia Butnaru
    • 1
  1. 1.University of Agricultural Sciences of Banat CountyTimisoaraRomania

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