Callus Formation and the Culturability of Inflorescence Primordia of Mutant Triticales
For a theoretical and practical reason the in vitro culture response of different Triticale genotyps “wild” and mutants was followed. The callusogenesis and culturability of spike explants were decisively influenced by the organogenetic stage.
The best growth medium was 66, MS supplemented with 2.4-D (1 mg/ 1) and Magnetic Particles (MP θ = 0.15 x 10-3 g/cm3) pH 5.8. Callusogenesis ability was positive on mutant genotypes (T. Tim 501-17 - an average 45.2%). The variability was higher at the “wild” regenerants. The FMR1 showed dwarfness (~9.7 cm; 40.4%) with small but normal plant characters. There were nuclear and plastone mutations also (tubular leaf, variegation and lethal chlorophyll deficiency).
The plant characters of FMR2, were more sharply differentiaded. The caulogenesis was strongly connected with the calli size (r = + 0.878).
KeywordsPlant Regeneration Callus Induction Magnetic Fluid Immature Embryo Mutant Genotype
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