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Response of Mature Triticale Embryos to Aluminium-Toxic Callus Induction Media

  • Claudia S. Gaus
  • Gitta Oettler
  • Claus-Ulrich Hesemann
Part of the Developments in Plant Breeding book series (DIPB, volume 5)

Abstract

Callus induction of threefold cut mature embryos of one winter triticale (x Triticosecale Wittmack) variety (“Lasko”) and three spring triticale genotypes (PFT 8512, Ocepar 1 and PFT 8710) was determined in a recently developed aluminium (Al)-toxic medium. Callus weight and pH alteration of spent medium were assessed at the end of the callus induction phase of 7 weeks in media containing different levels of AlCl3 (0.1 – 10mM) and being sterilised either by filter or by autoclaving.

In filter-sterilised media with concentrations higher than 2mM none of the four examined triticale genotypes developed calli and the pH-level of the spent medium remained nearly constant. Lower concentrations of 0.5mM and 1mM Al yielded callus weights significantly higher than the pH 3.6-control without AI, but less than the pH 4.6-control, and pH increased on the same level as in either control. In all concentrations below 2mM Al, somatic embryos could be observed. In contrast, the toxic effect of autoclaved Al-toxic media was expressed by inferior callus quality - even the controls were poor - and did not result in any somatic embryo.

Consequently, filter-sterilised Al-toxic media should be preferred and could serve to select Al-tolerant cell lines, e.g. in suspension cell cultures, or may be useful to investigate Al-tolerance mechanisms at the celluar level.

Keywords

Somatic Embryo Callus Induction Spend Medium Tepary Bean Toxic Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Claudia S. Gaus
    • 1
  • Gitta Oettler
    • 2
  • Claus-Ulrich Hesemann
    • 3
  1. 1.Research Centre Biotechnology and Plant BreedingUniversity of HohenheimStuttgartGermany
  2. 2.State Plant Breeding InstituteUniversity of HohenheimStuttgartGermany
  3. 3.Institute of GeneticsUniversity of HohenheimStuttgartGermany

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