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Matrix-Assisted Laser Desorption Mass Spectrometry on a Magnetic Sector Instrument Fitted With an Array Detector

  • R. S. Bordoli
  • K. Howes
  • R. G. Vickers
  • R. H. Bateman
  • D. J. Harvey
Part of the NATO ASI Series book series (ASIC, volume 475)

Abstract

Matrix assisted laser desorption ionisation (MALDI) has been carried out on a high performance double focusing mass spectrometer fitted with an integrating focal plane array detector. High sensitivity (low fmol detection) was obtained with peptides such as renin substrate. Underivatised complex oligosaccharides were also detectable at the 200 fmol level. The advantages of the array detector for improved resolution (5000 FWHM) and the benefits in complex oligosaccharide and glycolipid analyses relative to MALDI-TOF systems are shown. This improved resolution enabled ambiguities from the TOF data to be interpreted.

Keywords

Matrix Assisted Laser Desorption Ionisation Micro Channel Plate Renin Substrate Complex Oligosaccharide Focal Plane Array Detector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • R. S. Bordoli
    • 1
  • K. Howes
    • 1
  • R. G. Vickers
    • 1
  • R. H. Bateman
    • 1
  • D. J. Harvey
    • 2
  1. 1.Fisons Instruments, Organic Analysis, Analytical MSManchesterUK
  2. 2.Glycobiology Institute, Department of BiochemistryUniversity of OxfordOxfordUK

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