Conjugation in aquatic environments

  • Søren J. Sørensen
  • Niels Kroer
  • Erik Sørensen
  • Gitte Sengeløv
  • Tamar Barkay


Concerns with the horizontal exchange of genetic material by conjugation in aquatic environments arise from two issues. The first relates to conjugation as a mechanism that promotes genetic and physiological diversity in the aquatic microbial community [24]. The second arises from the need to regulate applications of genetically engineered microbes (GEMs) in environmental management practices [11, 14]. An understanding of conjugation in situ is needed to address both issues and this necessitates the development of experimental approaches and methods that enable following conjugal gene transfer among aquatic microbial populations in their natural habitats.


Recipient Strain Donor Strain Plasmid Transfer Conjugal Transfer Indigenous Bacterium 
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  1. 1.
    Altherr RM, Kasweck KL (1982) In situ studies with membrane diffusion chambers of antibiotic resistance transfer in Escherichia coli. Appl Environ Microbiol 44: 838–843.Google Scholar
  2. 2.
    Awong J, Britton G, Chaundry R (1990) Microcosms for assessing survival ofgenetically engineered microorganisms in aquatic environments. Appl Environ Microbiol 56: 977–983.Google Scholar
  3. 3.
    Bale MJ, Fry JC, Day MJ (1988) Transfer and occurrence of large mercury resistance plasmids in river epilithon. Appl Environ Microbiol 54: 972–978.Google Scholar
  4. 4.
    Barkay T, Gillman M, Liebert C (1990) Genes encoding mercuric reductases from selected gram-negative aquatic bacteria have a low degree of homology with merA of transposon Tn501. Appl Environ Microbiol 56: 1695–1701.Google Scholar
  5. 5.
    Barkay T, Liebert C, Gillman M (1993) Conjugal gene transfer to aquatic bacteria detected by the generation of a new phenotype. Appl Environ Microbiol 59: 807–814.Google Scholar
  6. 6.
    Barkay T, Kroer N, Rasmussen LD, Sørensen SJ (1995) Conjugal transfer at natural population densities in a microcosm simulating an estuarine environment. FEMS Microbiol Ecol 16: 43–54.CrossRefGoogle Scholar
  7. 7.
    De Lorenzo V, Herrero M, Jakubzik M, Timmis KN (1990) Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol 172: 6568–6572.Google Scholar
  8. 8.
    Fulthorpe RR, Wyndham RC (1992) Involvement of a chlorobenzoatecatabolic transposon, Tn5271, in community adaptation to chlorobiphenyl, chloroaniline, and 2,4-dichloro-phenoxyacetic acid in a freshwater ecosystem. Appl Environ Microbiol 58: 314–325.Google Scholar
  9. 9.
    Fulthorpe RR, Wyndham RC (1991) Transfer and expression of the catabolic plasmid pBRC60 in wild bacterial recipients in a freshwater ecosystem. Appl Environ Microbiol 57: 1546–1553.Google Scholar
  10. 10.
    Goodman AE, Hild E, Marshall KC, Hermansson H (1993) Conjugative plasmid transfer between bacteria under simulated marine oligotrophic conditions. Appi Environ Microbiol 59: 1035–1040.Google Scholar
  11. 11.
    Halvorson HO, Pramer D, Rogul M (1985) Engineered Organisms in the Environment: Scientific Issues. American Society for Microbiology, Washington D.C.Google Scholar
  12. 12.
    Hoagland DR, Arnon DI (1950) The Water Culture Method for Growing Plants without Soil. Circular 347. California Agricultural Experimental Station, Barkely, CA.Google Scholar
  13. 13.
    Jones GW, Baines L, Genthner FJ (1991) Heterotrophic bacteria of the freshwater neuston and their ability to act as plasmid recipients under nutrients deprived conditions. Microb Ecol 22: 15–25.CrossRefGoogle Scholar
  14. 14.
    Klingmüller W (1988) Risk Assessment for Deliberate Releases: The Possible Impact of Genetically Engineered Microorganisms on the Environment. Springer-Verlag KG, Berlin.CrossRefGoogle Scholar
  15. 15.
    Kroer N, Coffin RB (1992) Microbial trophic interactions in aquatic microcosms designed for testing genetically engineered microorganisms: A field comparison. Microb Ecol 23: 143–157.CrossRefGoogle Scholar
  16. 16.
    Kroer N, Coffin RB, Jørgensen NOJ (1994) Comparison of microbial trophic interactions in aquatic microcosms designed for the testing of introduced microorganisms. Environ Tox Chem 13: 247–257.CrossRefGoogle Scholar
  17. 17.
    Levy SB, Miller RV (1989) Gene Transfer in the Environment. McGraw-Hill Publishers Co., New York.Google Scholar
  18. 18.
    Liang LL, Sinclair JL, Mallory LM, Alexander M (1982) Fate in model ecosystems of microbial species of potential use in genetic engineering. Appl Environ Microbiol 44: 708–714.Google Scholar
  19. 19.
    Mancini P, Fertels S, Nave D, Gealt MA (1987) Mobilization of plasmid pHSV106 from Escherichia coli HB101 in a laboratory-scale waste treatment facility. Appl Environ Microbiol 53: 665–671.Google Scholar
  20. 20.
    Munro PM, Gauthier, MJ Lamond FM (1987) Changes in Echerichia coli cells starved in seawater or grown in seawater-wastewater mixtures. Appl Environ Microbiol 53: 1476–1488.Google Scholar
  21. 21.
    O’Morchoe SB, Ogunseitan O, Sayler GS, Miller RV (1988) Conjugal transfer of R68.45 and FP5 between Pseudomonas aeruginosa strains in a freshwater environment. Appl Environ Microbiol 54: 1923–1929.Google Scholar
  22. 22.
    Pettibone GW, Sullivan SA, Shiaris HP (1987) Comparative survival of antibiotic-resistant and sensitive fecal indicator bacteria in estuarine water. Appl Environ Microbiol 53: 1241–1245.Google Scholar
  23. 23.
    Pritchard PH, Bourquin AW (1984) The use of microcosms for evaluation of interactions between pollutants and microorganisms. Adv Microb Ecol 7: 133–215.CrossRefGoogle Scholar
  24. 24.
    Reanney DC, Gowland PC, Slater H (1983) Genetic interactions among microbial communities. In: Bull AT, Slater JH (eds) Microbial Interactions and Communities, Vol. 1, pp. 379–421. Academic Press Inc., New York.Google Scholar
  25. 25.
    Rochelle PA, Fry JC, Day MJ (1989) Factors affecting conjugal transfer of plasmids encoding mercury resistance from pure cultures annd mixed natural suspensions of epilithic bacteria. J Gen Microbiol 135: 409–424.Google Scholar
  26. 26.
    Sandaa RA, Enger Ø (1994) Transfer in marine sediments of the naturally occurring plasmid pRASl encoding multiple antibiotic resistance. Appl Environ Microbiol 60: 4234–4238.Google Scholar
  27. 27.
    Saouter E, Gillman M, Turner R, Barkay T (1995) Development of field validation of a microcosm to simulate the mercury cycle in a contaminated pond. Environ Toxicol Chem 14: 69–77.CrossRefGoogle Scholar
  28. 28.
    Scanferlato VS, Orvos DR, Cairns Jr J, Lacy GH (1989) Genetically engineered Envinia carotovora in aquatic microcosms: Survival and effects on functional groups of indigenous bacteria. Appl Environ Microbiol 55: 1477–1482.Google Scholar
  29. 29.
    Silver S, Walderhaug M (1992) Gene regulation of plasmid-and chromosome-determined inorganic ion transport in bacteria. Microbiol Rev 56: 195–228.Google Scholar
  30. 30.
    Sinclair JL, Alexander M (1984) Role of resistance to starvation in bacterial survival in sewage and lake water. Appl Environ Microbiol 48: 410–415.Google Scholar
  31. 31.
    Smit E, Van Elsas JD (1990) Determination of plasmid transfer frequency in soil: Consequences of bacterial mating on selective agar media. Curr Microbiol 21: 151–157.CrossRefGoogle Scholar
  32. 32.
    Smit E, Van Elsas JD, Van Veen JA, De Vos WM (1991). Detection of plasmid transfer from Pseudomonas fluorescens to indigenous bacteria in soil by using bacteriophage fR2f for donor counterselection. Appl Environ Microbiol 57: 3482–3488.Google Scholar
  33. 33.
    Sobecky PA, Schell MA, Moran MA, Hodson RE (1992) Adaptation of model genetically engineered microorganisms to lake water: growth rate enhancements and plasmid loss. Appl Environ Microbiol 58: 3630–3637.Google Scholar
  34. 34.
    Summers AO, Barkay T (1989) Metal resistance genes in the environment. In: Levy SB, Miller RV (eds) Gene Transfer in the Environment, pp. 287–308. McGraw-Hill Publishing Co., New York.Google Scholar
  35. 35.
    Sundin GW, Demezes DH, Bender CL (1994) Genetic and plasmid diversity within natural populations of Pseudomonas syringae with various exposures to copper and streptomycin bactericides. Appl Environ Microbiol 60: 4421–4431.Google Scholar
  36. 36.
    Sørensen SJ (1991) Survival of Escherichia coli K12 in seawater. FEMS Microbiol Ecol 85: 161–168.CrossRefGoogle Scholar
  37. 37.
    Sørensen SJ (1992) Mobilization of non-conjugative pBR322-derivative plasmids from laboratory strains of Escherichia coli to bacteria isolated from seawater. Microb Releases 1: 17–22.Google Scholar
  38. 38.
    Sørensen SJ (1993) Transfer of plasmid RP4 from E. coli K12 to indigenous bacteria in seawater. Microb Releases 2: 135–141.Google Scholar
  39. 39.
    Sørensen SJ, Barkay T (1991) Experimental approach for the detection of gene transfer from GEM’s to bacteria indigenous to aquatic environments. 3rd Symposium on Bacterial Genetics and Ecology. Villefranche sur Mer, France, Nov. 20–22, 1991.Google Scholar
  40. 40.
    Top E, De Smet I, Verstraete W, Dijkmans R, Mergeay M (1994) Exogenous isolation of mobilizing plasmids from polluted soils and sludges. Appl Environ Microbiol 60: 831–839.Google Scholar
  41. 41.
    Van der Meer JR, De Vos WM, Harayama S, Zehnder AJB (1992) Molecular mechanisms of genetic adaptation to xenobiotic compounds. Microbiol Rev 56: 254–259.Google Scholar
  42. 42.
    Wilkins B, Lanka E (1993) DNA prosessing and replication during plasmid transfer between gram-negative bacteria. In: Clewell DB (ed) Bacterial Conjugation, pp. 105–136. Plenum Press, New York.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1996

Authors and Affiliations

  • Søren J. Sørensen
    • 1
  • Niels Kroer
    • 2
  • Erik Sørensen
    • 1
  • Gitte Sengeløv
    • 1
  • Tamar Barkay
    • 3
  1. 1.Dept. of General MicrobiologyUniversity of CopenhagenCopenhagen KDenmark
  2. 2.Dept. of Marine Ecology and MicrobiologyNational Environmental Research InstituteRoskildeDenmark
  3. 3.Microbial Ecology and Biotechnology BranchUS Environmental Protection AgencyGulf BreezeUSA

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