Advertisement

Subtraction hybridization for the production of high specificity DNA probes

  • A. J. Bjourson
  • J. E. Cooper

Abstract

Microbes can be exploited in the environment to improve plant yields by symbiotic nitrogen fixation or as biological control agents. It is desirable to monitor the fate of released microorganisms in order to evaluate their survival, the success of inoculation, or to study their effect on other microorganisms. In epidemiological and ecological studies also, it is important to identify species and strains of organisms accurately.

Keywords

Genomic Subtraction Large Molar Excess Probe Strain Subtracter Sequence Improve Plant Yield 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bjourson AJ, Cooper JE (1988) Isolation of Rhizobium loti strain-specific DNA sequences by subtraction hybridization. Appl Environ Microbiol 53: 1705–1707.Google Scholar
  2. 2.
    Bjourson AJ, Stone CE, Cooper JE (1992) Combined subtraction hybridization and polymerase chain reaction amplification procedure for the isolation of strain specific Rhizobium DNA sequences. Appl Environ Microbiol 58: 2296–2301.Google Scholar
  3. 3.
    Drahos DJ (1991) Methods for the detection, identification and enumeration of microbes. In: Andrews JH, Hirano SS (eds) Microbial Ecology of Leaves, pp. 135–158. Springer Publishers, New York.CrossRefGoogle Scholar
  4. 4.
    Perret X, Fellay R, Bjourson AJ, Cooper JE, Brenner S, Broughton WJ (1994) Subtraction hybridization and shot-gun sequencing: a new approach to identify symbiotic loci. Nucl Acids Res 22: 1335–1341.CrossRefGoogle Scholar
  5. 5.
    Scott MRD, Westphal K-W, Rigby PWC (1983) Activation of mouse genes in transformed cells. Cell 34: 557–567.CrossRefGoogle Scholar
  6. 6.
    Sive HL, St John T (1988) A simple subtractive hybridization technique employing photoactive biotin and phenol extraction. Nucl Acids Res 16: 10937.CrossRefGoogle Scholar
  7. 7.
    Straus D, Ausubel FM (1990) Genomic subtraction for cloning DNA corresponding to deletion mutants. Proc Natl Acad Sci USA 87: 1889–1892.CrossRefGoogle Scholar
  8. 8.
    Streit W, Bjourson AJ, Cooper JE, Werner D (1993) Application of subtraction hybridization for the development of a Rhizobium leguminosarum biovar phaseoli and Rhizobium tropici group-specific DNA probe. FEMS Microbiol Ecol 13: 59–68.CrossRefGoogle Scholar
  9. 9.
    Sun T-P, Goodman HM, Ausubel FM (1992) Cloning of the Arabidopsis GA1 locus by genomic subtraction. Plant Cell 4: 119–128.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1996

Authors and Affiliations

  • A. J. Bjourson
    • 1
  • J. E. Cooper
    • 1
  1. 1.Department of Applied Plant ScienceThe Queen’s University of BelfastBelfastNorthern Ireland

Personalised recommendations