Abstract
While diagnostics based on the polymerase chain reaction (PCR) are being applied actively in medical and forensic studies, much less development has occurred with respect to plant pathology and other organisms in the environment (for review see [11]). Despite the many obvious opportunities, applications often have been retarded significantly by the special problems that are associated with such samples, namely inhibitors of the PCR reactions which are frequently present in plant and soil extracts and can severely retard or completely prevent PCR amplification.
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© 1996 Springer Science+Business Media Dordrecht
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Nazar, R.N., Robb, E.J., Volossiouk, T. (1996). Direct extraction of fungal DNA from soil. In: Akkermans, A.D.L., Van Elsas, J.D., De Bruijn, F.J. (eds) Molecular Microbial Ecology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0215-2_1
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DOI: https://doi.org/10.1007/978-94-009-0215-2_1
Publisher Name: Springer, Dordrecht
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