Advertisement

The biochemistry of methanol dehydrogenase

  • Christopher Anthony
  • Simon L. Dales

Abstract

Methanol dehydrogenase (MDH) is responsible for oxidation of methanol to formaldehyde in almost all bacteria growing aerobically on methane or methanol; it is a periplasmic quinoprotein which passes its electrons to a specific c-type cytochrome which is usually called cytochrome c L (Anthony, 1986, 1992, 1993, Goodwin and Anthony, 1995). Our understanding of this enzyme has increased dramatically in the last 3 years with publication of the 3-dimensional structures of the enzyme from Methylotrophus W3A1 (at 2.6 Å) (Xia et al., 1992; White et al., 1993) and from Methylobacterium extorquens (at 1.94Å) (Anthony et al., 1994; Ghosh et al., 1995). The system considered in this brief review will be the MDH and cytochrome c L from M. extorquens; in particular, the following questions will be considered: what does the structure tell us about the mechanisms of methanol oxidation, and electron transfer between the two proteins, and the initial interaction (‘docking’) between MDH and cytochrome c L.

Keywords

Methanol Oxidation Prosthetic Group Acetic Acid Bacterium Disulphide Bridge Paracoccus Denitrificans 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anthony, C. & Zatman, L.J. (1964) Biochem. J.92, 614 – 621.PubMedCentralPubMedGoogle Scholar
  2. Anthony, C. (1986) Adv. Microbial. Physiol.27, 113 – 210.CrossRefGoogle Scholar
  3. Anthony, C. (1992) Biochim. Biophys. Acta 1099, 1 – 15.Google Scholar
  4. Anthony, C. (1993) inPrinciples and applications of quinoproteins(Davidson, V.L. ed.), Methanol dehydrogenase in Gram-negative bacteria, pp. 17–45, Marcel Dekker, New York.Google Scholar
  5. Anthony, C., Ghosh, M. & Blake, C.C.F. (1994) Biochem. J.304 665–674.PubMedCentralPubMedGoogle Scholar
  6. Avezoux, A., Goodwin, M.G. & Anthony, C. (1995) Biochem. J.307 735–741.PubMedCentralPubMedGoogle Scholar
  7. Blake, C.C.F., Ghosh, M., Harlos, K., Avezoux, A. & Anthony, C. (1994) Nature Struct Biol. 1, 102 – 105.PubMedCrossRefGoogle Scholar
  8. Chan, H.T.C. & Anthony, C. (1991) Biochem. J. 280, 139 – 146.PubMedCentralPubMedGoogle Scholar
  9. Chan, H.T.C. & Anthony, C. (1992) FEMS Microbiol. Lett.96, 231 – 234.CrossRefGoogle Scholar
  10. Chen, L.Y., Mathews, F.S., Davidson, V.L., Huizinga, E.G., Vellieux, F.M.D. & Hol, W.G.J. (1992) Protein-Struct. Funct. Genet.14, 288 – 299.Google Scholar
  11. Cox, J.M., Day, D.J. & Anthony, C. (1992) Biochim. Biophys. Acta1119 7–106.Google Scholar
  12. Cozier, G.E., Giles, I.G. & Anthony, C. (1995) Biochem. J.307 375 – 379.Google Scholar
  13. Dales, S.L. & Anthony, C. (1995) Biochem. J. In Press.Google Scholar
  14. Dijkstra, M., Frank, J. & Duine, J.A. (1989) Biochem. J.257 87 – 94.PubMedCentralPubMedGoogle Scholar
  15. Frank, J., Dijkstra, M., Duine, AJ. & Balny, C. (1988) Eur. J. Biochem.174 331–338.Google Scholar
  16. Frank, J., van Krimpen, S.H., Verwiel, P.E.J., Jongejan, J.A., Mulder, A.C. & Duine, J.A. (1989) Eur. J. Biochem.184, 187–195.PubMedCrossRefGoogle Scholar
  17. Ghosh, M., Anthony, C., Harlos, K., Goodwin, M.G. & Blake, C.C.F. (1995). Structure3, 177 – 187.PubMedCrossRefGoogle Scholar
  18. Goodwin, P.M. & Anthony, C. (1995) Microbiology141, 1051 – 1064.PubMedCrossRefGoogle Scholar
  19. Harris, T.K. & Davidson, V.L. (1993) Biochemistry32 14145 – 14150.PubMedCrossRefGoogle Scholar
  20. Harris, T.K., Davidson, V.L., Chen, L.Y., Mathews, F.S. & Xia, Z.X. (1994) Biochemistry 33, 12600 – 12608.PubMedCrossRefGoogle Scholar
  21. Ito, N., Phillips, S.E.V., Yadav, K.D.S. & Knowles, P.F. (1994) J. Mol. Biol.238, 794 – 814.PubMedCrossRefGoogle Scholar
  22. Long, A.R. & Anthony, C. (1991) J. Gen. Microbiol.137, 415–425.CrossRefGoogle Scholar
  23. Matsushita, K., Shinagawa, E., Adachi, O. & Ameyama, M. (1989) J. Biochem.105, 633–637.PubMedGoogle Scholar
  24. Richardson, I.W. & Anthony, C. (1992) Biochem. J.287, 709 – 715.PubMedCentralPubMedGoogle Scholar
  25. White, S., Boyd, G., Mathews, F.S., Xia, Z.X., Dai, W.W., Zhang, Y.F. & Davidson, V.L. (1993) Biochemistry32, 12955–12958.PubMedCrossRefGoogle Scholar
  26. Xia, Z.X., Dai, W.W., Xiong, J.P., Hao, Z.P., Davidson, V.L., White, S. & Mathews, F.S. (1992) J. Biol. Chem.267, 22289 – 22297.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Christopher Anthony
    • 1
  • Simon L. Dales
    • 1
  1. 1.Biochemistry DepartmentUniversity of SouthamptonUK

Personalised recommendations