Analysis of genes in the pathway for the fermentation of acetate to methane by Methanosarcina thermophila

  • J. G. Ferry
  • J. A. Maupin-Furlow


Most acetotrophic anaerobes from the “Bacteria” domain cleave acetate and then oxidize the methyl and carbonyl groups to CO2 and reduce exogenous electron acceptors. Acetate-utilizing methane-producing “Archaea” also cleave acetate; however, the carbonyl group is oxidized to CO2 to provide an electron pair for reduction of the methyl group to CH4. The phylogenetic extremes between the two domains raises questions concerning the comparative genetics and biochemistry of acetate utilization in diverse anaerobes.


Bacterium Domain Acetate Kinase Acetyl Phosphate psmB Gene Nuclease Protection Assay 
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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • J. G. Ferry
    • 1
  • J. A. Maupin-Furlow
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyPennsylvania State UniversityUniversity ParkUSA

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