The Implication of pH on the Design of an Enzymatic Reactor for Penicillin Hydrolysis

  • Alfonso Gomez-Aguirre
  • Rodolfo R. Quintero
  • Agustin Lopez-Munguia
Part of the NATO ASI Series book series (NSSE, volume 305)


In this paper penicillin acylase is used to exemplify the effect of pH on the design of enzymatic reactors. Simulation programs are used to describe the batch, continuous stirred tank, packed bed and recirculated reactors using a triple inhibition model for enzyme kinetics, an electrochemical model to describe the pH changes with conversion and an Arrhenius type equation to account for the pH effect on enzyme stability.


Batch Reactor Continuous Stir Tank Reactor Penicillin Acylase Arrhenius Type Equation Recirculation Mode 
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  1. 1.
    Pitcher W.H., 1980. Immobilized Enzymes for food processing C.R.C. Press., Boca Raton, Florida.Google Scholar
  2. 2.
    Weetall H.H. & Pitcher W.H., 1986.Scaling up an Immobilized Enzyme System. Science, 232, 1396–1402.CrossRefGoogle Scholar
  3. 3.
    Martinez Espindola J.P. & Lopez-Munguia C.A., 1985. On the kinetics of dextransucrase and dextran synthesis in batch reactors Biotechnol. Lett. 7,7, 483–486.CrossRefGoogle Scholar
  4. 4.
    Klein J., Vorlop K.D.J. Wagner F., 1984. The implication of reaction kinetics and mass transfer on the design of biocatalytic processes with immobilized cells. Ann. N.Y. Acad. Sci. 434, 437–449.CrossRefGoogle Scholar
  5. 5.
    Carleysmith S.W. & Lilly M., 1979. Deacylation of benzylpenicillin by immobilized penicillin acylase in a continuous four stage stirred tank reactor. Biotechnol. Bioeng., 21, 1057–1073.CrossRefGoogle Scholar
  6. 6.
    Mollgaard H.,1987. Choice of reactor for semacylase. Ann. N.Y. Acad. Sci. 501, 473–476.CrossRefGoogle Scholar
  7. 7.
    Cardoso J.P. & Bento Correia M., 1986. Modelling of the hydrolysis of benzylpenicillin to 6-APA and PAA by an immobilized penicillin acylase in a small pilot plant bench recirculated reactor. British Polym. J., 18, 5, 323–332.CrossRefGoogle Scholar
  8. 8.
    Ospina S., Lopez-Munguia A., Gonzalez R.L. & Quintero R., 1992. Characterization and use of a penicillin acylase biocatalyst. J. Chem. Technol. & Biotechnol., 53. In press.Google Scholar
  9. 9.
    Gomez-Aguirre A., Ospina S., Quintero R. & Lopez-Munguia A, 1992. Modelling and simulation of a pH dependent bioprocess enzymatic conversion of Penicillin G to 6-APA. In: Bioprocess commutations in Biotechnology Vol. II, Ed. T.Ghose. Ellis Horwood, UK. In Press.Google Scholar
  10. 10.
    Ospina S., 1991. Characterization and application of a penicillin acylase biocatalyst. M Sc Thesis. Inst, of Biotechnology, UNAM, Mexico D.F.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Alfonso Gomez-Aguirre
    • 1
  • Rodolfo R. Quintero
    • 2
  • Agustin Lopez-Munguia
    • 2
  1. 1.Instituto de BiotecnologiaUniversidad Nacional de ColombiaBogotaColombia
  2. 2.Instituto de BiotecnologiaUniversidad Nacional Autonoma de MexicoCuernavacaMexico

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