Chemical fusion of protoplasts

  • P. Anthony
  • R. Marchant
  • N. W. Blackhall
  • J. B. Power
  • M. R. Davey


Somatic hybridisation of plants offers a novel method of circumventing sexual barriers in plant breeding. It not only permits the production of new hybrids between sexually incompatible genotypes [14], but also serves as a method for the genetic modification of vegetatively propagated crops, sterile or subfertile species and plants with relatively long life cycles [5], such as tree species. Somatic hybridisation involves four distinct stages; protoplast isolation, protoplast fusion, selection and plant regeneration followed by cytological and molecular analysis of regenerated plants.


Somatic Hybrid Protoplast Fusion Protoplast Suspension Somatic Hybrid Plant Protoplast System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Ahuja PS, Laiq-ur-Rahman, Bhargava SC, Banerjee S (1993) Regeneration of somatic hybrid plants between Atropa belladonna L. and Hyoscyamus muticus L. Plant Sci 92: 91–98.CrossRefGoogle Scholar
  2. 2.
    Boni LT, Hui SW (1987) The mechanism of polyethylene glycol-induced fusion in model membranes. In: Sowers AE (Ed.) Cell Fusion, pp 119–124, Plenum Press, New York.Google Scholar
  3. 3.
    Chand PK, Davey MR, Power JB, Cocking EC (1988) An improved procedure for protoplast fusion using polyethylene glycol. J Plant Physiol 133: 480–485.CrossRefGoogle Scholar
  4. 4.
    Craig AL, Morrison I, Baird E, Waugh R, Coleman M, Davie P, Powell W (1994) Expression of reducing sugar accumulation in interspecific somatic hybrids of potato. Plant Cell Rep 13: 401–405.CrossRefGoogle Scholar
  5. 5.
    Gleba YY, Sytnik KM (1984) Protoplast fusion and parasexual hybridisation of higher plants. In: Shoeman R (Ed.) Protoplast Fusion, pp 36–62, Springer-Verlag, Berlin.Google Scholar
  6. 6.
    Hammatt N, Lister A, Blackball NW, Gartland J, Ghose TK, Gilmour DM, Power JB, Davey MR, Cocking EC (1990) Selection of plant heterokaryons from diverse origins by flow cytometry. Protoplasma 154: 34–44.CrossRefGoogle Scholar
  7. 7.
    Hammatt N, Blackhall NW, Davey MR (1991) Variation in the DNA content of Glycine species. J Exp Bot 42: 659–665.CrossRefGoogle Scholar
  8. 8.
    Kishinami I, Widholm F (1987) Auxotrophic complementation in intergeneric hybrid cells obtained by electrical and dextran-induced protoplast fusion. Plant Cell Physiol 28: 211–218.Google Scholar
  9. 9.
    Louzada ES, Grosser JW, Gmitter FG (1993) Intergeneric somatic hybridisation of sexually incompatible parents: Citrus sinensis and Atalantia ceylanica. Plant Cell Rep 12: 687–690Google Scholar
  10. 10.
    Mendis MH, Power JB, Davey MR (1991) Somatic hybrids of the forage legumes Medicago sativa L. and M. falcata L. J Exp Bot 42: 1565–1573.Google Scholar
  11. 11.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.CrossRefGoogle Scholar
  12. 12.
    Nagata T (1978) A novel cell fusion method of protoplasts by polyvinyl alcohol. Naturwissenschaften 65: 263–264.CrossRefGoogle Scholar
  13. 13.
    Nakano M, Mii M (1993) Interspecific somatic hybridisation in Dianthus: selection of hybrids by the use of iodoacetamide inactivation and regeneration ability. Plant Sci 88: 203–208.CrossRefGoogle Scholar
  14. 14.
    Patil RS, Latif M, Vaz FBD, Davey MR, Power JB (1993) Hybridisation, through culture of embryos and immature seeds, of a range of tomato cultivars with a tomato somatic hybrid (Lycopersicon esculentum (+) L. peruvianum): emergence of a possible new marker gene for tomato breeding. Plant Breed 111: 273–282.CrossRefGoogle Scholar
  15. 15.
    Pehu E, Thomas M, Poutala T, Karp A, Jones MGK (1990) Species-specific sequences in the genus Solanum - identification, characterisation and application to study somatic hybrids of Solanum brevidens and Solanum tuberosum. Theor App Genet 80: 693–698.Google Scholar
  16. 16.
    Polgar Z, Preiszner J, Dudits D, Feher A (1993) Vigorous growth of fusion products allows highly efficient selection of interspecific potato somatic hybrids–molecular proofs. Plant Cell Rep 12: 399–402.Google Scholar
  17. 17.
    Power JB, Cummins SE, Cocking EC (1970) Fusion of isolated plant protoplasts. Nature 225: 1016–1018.PubMedCrossRefGoogle Scholar
  18. 18.
    Power JB, Frearson EM, Haywood C, George D, Evans PK, Berry SF, Cocking EC (1976) Somatic hybridisation of Petunia hybrida and R. parodii. Nature 263: 500–502.Google Scholar
  19. 19.
    Schoenmakers HCH, Wolters AMA, Nobel EM, de Klein CMJ, Koornneef M (1993) Allotriploid somatic hybrids of diploid tomato (Lycoperiscon esculentum Mill.) and monoploid potato (Solanum tuberosum L.). Theor Appl Genet 87: 328–336.CrossRefGoogle Scholar
  20. 20.
    Schoenmakers HCH, Wolters AMA, de Haan A, Saiedi AK, Koornneef M (1994) Asymmetric somatic hybridisation between tomato (Lycopersicon esculentum Mill) and gamma-irradiated potato (Solanum tuberosum L.): a quantitative analysis. Theor Appl Genet 87: 713–720.CrossRefGoogle Scholar
  21. 21.
    Stattmann M, Gerick E, Wenzel G (1994) Interspecific somatic hybrids between Solanum khasianum and S. aculeatissimum produced by electrofusion. Plant Cell Rep 13: 193–196.Google Scholar
  22. 22.
    Wang GR, Binding H (1993) Somatic hybridisation between Senecio fuchsii and S. jacobaea. Acta Hort 36: 315–320Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • P. Anthony
    • 1
  • R. Marchant
    • 1
  • N. W. Blackhall
    • 1
  • J. B. Power
    • 1
  • M. R. Davey
    • 1
  1. 1.Plant Genetic Manipulation Group, Department of Life ScienceUniversity of NottinghamNottinghamUK

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