Somatic hybridisation of plants offers a novel method of circumventing sexual barriers in plant breeding. It not only permits the production of new hybrids between sexually incompatible genotypes , but also serves as a method for the genetic modification of vegetatively propagated crops, sterile or subfertile species and plants with relatively long life cycles , such as tree species. Somatic hybridisation involves four distinct stages; protoplast isolation, protoplast fusion, selection and plant regeneration followed by cytological and molecular analysis of regenerated plants.
KeywordsSomatic Hybrid Protoplast Fusion Protoplast Suspension Somatic Hybrid Plant Protoplast System
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