Isolation and uptake of plant nuclei

  • Praveen K. Saxena
  • John King

Abstract

Genetic transformation of animal cells by transplantation of isolated organelles, nuclei, and chromosomes is well documented and has played a significant role in investigating chromosome mapping and the regulation of gene expression [11, 13]. In plants, direct DNA transfer using isolated organelles was attempted following the discovery of techniques to eliminate the plant-specific barrier, the cell wall, which previously hampered the introduction of organelles into cells. Predictably, as soon as plant cells succumbed to procedures capable of enzymic degradation of cell walls, a whole new concept of handling naked plant cells (protoplasts) emerged as well as a genuine hope of being able to transform plant cells by foreign DNA introduction [15]. Thus, many workers studied the uptake of a variety of macromolecules and demonstrated the ability of plant protoplasts to accept foreign particles such as ferritin, bacteria, and isolated organelles like nuclei and chloroplasts (see references 7, 14, 21 for extensive reviews).

Keywords

Cell Suspension Culture Nuclear Transplantation Plant Protoplast Somatic Cell Genetic Nuclear Uptake 
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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Praveen K. Saxena
    • 1
  • John King
    • 2
  1. 1.Department of Horticultural ScienceUniversity of GuelphGuelphCanada
  2. 2.Department of BiologyUniversity of SaskatchewanSaskatoonCanada

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