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Clonal propagation of eucalypts

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Plant Tissue Culture Manual

Abstract

The recent Flora of Australia [14] lists 513 species of eucalypts and some authorities consider they should be split into several separate genera. Consequently a review of micropropagation techniques for eucalypts must necessarily be more general than an account directed towards a single species. More than 50 species and many hybrids have been grown in vitro. Several reviews [24, 40, 50, 70] tabulate the media and the levels of success achieved with these species. Despite the diversity of the species it is possible to suggest media and techniques which should result in sufficient in vitro material with which to do meaningful experiments on optimising media and conditions.

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References

  1. Adam S, Chrisqui D, David C (1987) Involvement of the physiological state of Eucalyptus gunnii in its ability to insert T–DNA of Agrobacterium rhizogenes. XIVth Bot Congress Berlin July 24–Aug 1 1987. Abstract 3–113–1, pp 461.

    Google Scholar 

  2. Bachelard EP, Stowe BB (1963) Growth in vitro of roots of Acer rubrum L. and Eucalyptus camaldulensis Dehn. Physiol Plant 16: 20–30.

    Article  CAS  Google Scholar 

  3. Bell DT, van der Moezel P, Bennett IJ, McComb JA, Wilkins C, Marshall S, Morgan A (1993a) Comparisons of growth of Eucalyptus camaldulensis from seeds and tissue culture: root, shoot and leaf morphology of 9-month-old plants grown in deep sand and sand over clay. For Ecol Manag 57: 125–139.

    Article  Google Scholar 

  4. Bell DT, McComb JA, Van der Moezel PG, Bennett IJ, Kabay ED (1993b) Comparisons of selected cloned plantlets against unselected seedlings for rehabilitation of waterlogged and saline discharge zones in Australian agricultural catchments. Aust For (in press).

    Google Scholar 

  5. Bennett IJ (1988) Use of Tissue Culture Techniques for the Improvement of Eucalyptus marginata Donn ex Sm. PhD thesis Murdoch University, Western Australia.

    Google Scholar 

  6. Bennett IJ, Tonkin CM, Wroth MM, Davison EM, McComb JA (1986) A comparison of the growth of seedling and micropropagated Eucalyptus marginata (jarrah). Early growth to 2 years. For Ecol Manag 14: 1–12.

    Article  Google Scholar 

  7. Bennett IJ, McComb JA, Bradley JS (1992a) Testing the expression of resistance to insect attack: resistance of jarrah (Eucalyptus marginata Donn ex Sm) to jarrah leaf miner (Perthida glyphopa Common). For Ecol Manag 48: 99–105.

    Article  Google Scholar 

  8. Bennett IJ, McComb JA, Tonkin CM, McDavid D (1992b) Effect of cytokinnins on multiplication and rooting of Eucalyptus globulus and other Eucalyptus species. In: AFOCEL Mass Production Technology for Genetically Improved Fast Growing Forest Tree Species. AFOCEL Bordeaux France Sept 14 1992 2: 195–201.

    Google Scholar 

  9. Bennett IJ, McComb JA, Tonkin CM, McDavid DAJ (1993) Alternating cytokinins in multiplication media stimulates in vitro shoot growth and rooting of Eucalyptus globulus Labill. Ann Bot (in press).

    Google Scholar 

  10. Benson EE (1990) Free radicals in stressed and aging plant tissue cultures. In Rodriquez R (Eds.) Plant Aging: Basic and Applied Approaches, pp 269–275, Plenum Publ, New York.

    Google Scholar 

  11. Blomstead C, Cameron J, Whiteman P, Chandler S (1991). Micropropagation of juvenile Eucalyptus regnans mountain ash. Aust J Bot 39: 179–186.

    Article  Google Scholar 

  12. Boulay M (1983) Micropropagation of frost resistant Eucalyptus. In: Proc Workshop on Eucalyptus in California. June 14–16, Sacramento California.

    Google Scholar 

  13. Boxus P, Terzi JM, Lievins C, Pylyser M, Ngaboyamahina P, Duhem K (1991) Improvement and perspectives of micropropagation techniques applied to some hot climate plants. Acta Hort 289: 55–64.

    Google Scholar 

  14. Bureau of Flora and Fauna (1987) Flora of Australia Vol 19 Myrtaceae - Eucalyptus Angophora. (Aust Publ Service Canberra).

    Google Scholar 

  15. Carter A, Slee MU (1991) Etiolation as an aid to rooting cuttings of Eucalyptus grandis. In: IUFRO Symposium on Intensive Forestry: The Role of Eucalypts (Durban South Africa Sept 1991 ) 1: 64–69.

    Google Scholar 

  16. Carvalho DD, Pinto JEBP, Pasqual M (1990) Use of fungicides and antioxidants for in vitro nodal segments culture of Eucalyptus grandis Hill ex Maiden. Cient Prat. 14: 97–106 (in Portuguese).

    Google Scholar 

  17. Chang SH, Donald DEM, Jacobs G (1992) Micropropagation of Eucalyptus radiata spp radiata using explants from mature and coppice material. S Afr For J 162: 43–47.

    Google Scholar 

  18. Cheng B, Peterson CM, Mitchell RJ (1992) The role of sucrose, auxin and explant source on the in vitro rooting of seedling explants of Eucalyptus sideroxylon. Plant Science 87: 207–214.

    Article  CAS  Google Scholar 

  19. Cresswell R, de Fossard R (1974) Organ culture of Eucalyptus grandis. Aust For 37: 55–69.

    Article  Google Scholar 

  20. Cresswell R, Nitch C (1975) Organ culture of Eucalyptus grandis L. Planta 125: 87–90.

    Article  Google Scholar 

  21. Curir P, van Sumere CF, Termini A, Barthe P, Marchesini A, Doki M (1989) Flavonoid accumulation is correlated with adventitious roots formation. In: Eucalyptus gunnii Hook. micropropagated through axillary bud stimulation. Plant Physiol 92: 1148–1153.

    Google Scholar 

  22. Dhawan AK, Paton DM, Willing RR (1979) Occurrence and bioassay responses of G, a plant growth regulator in Eucalyptus and other Myrtaceae. Planta 146: 419–22.

    Article  CAS  Google Scholar 

  23. Doran JC, Carter AS, Matheson AC (1992) Variation in root strike of Petford Eucalyptus camaldulensis clones. In: AFOCEL Mass Production Technology for Genetically Improved Fast Growing Forest Tree Species. AFOCEL Bordeaux France Sept 14 1992 1: 407–414.

    Google Scholar 

  24. Durand-Creswell R, Boulay M, Franclet A (1982) Vegetative propagation of Eucalyptus. In: Bonga JM, Durzan D (Eds.) Tissue Culture in Forestry, Martinus Nijhoff, The Hague.

    Google Scholar 

  25. Falkiner (1990) The criteria for choosing an antibiotic for control of bacteria in plant tissue culture. Intern Assoc Pl Tissue Culture Newsletter No 60 13–23.

    Google Scholar 

  26. Franclet A (1991) Biotechnology in `rejuvenation’: hope for the micropropagation of difficult woody plants. Acta Hort 289: 273–282.

    Google Scholar 

  27. Franclet A, Boulay M, Bekkaoui F, Fouret Y, Vershoore-Marouzet B, Walker N (1987) Rejuvenation. In: Bonga J, Durzan D (Eds.) Cell and Tissue Culture in Forestry 1: 232–248.

    Chapter  Google Scholar 

  28. Furuya T, Orihara Y, Miyatake H (1989) Biotransformation of (-) methanol by Eucalyptus perriniana cultured cells. J Chem Soc Perkin Trans 1: 1171–1719.

    Google Scholar 

  29. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158.

    Article  PubMed  CAS  Google Scholar 

  30. Gupta PK, Mascarenhas AF, Jagannathan V (1981) Tissue culture of forest trees–clonal propagation of mature trees of Eucalyptus citriodora Hook. by tissue culture. PI Sci Lett 20: 195–201.

    Article  Google Scholar 

  31. Hartney V (1982) Tissue culture of Eucalyptus. Comb Proc Intern Pl Prop Soc 32: 98–109.

    Google Scholar 

  32. Hartney V, Barker PK (1980) The vegetative propagation of eucalypts by tissue culture. In: Fast Growing Trees, IUFRO Congress Brazil.

    Google Scholar 

  33. Hyndman SE, Hasegana PM, Bressan RA (1982) The role of sucrose and nitrogen in adventitious root formation of cultured rose shoots. Pl Cell Tissue and Organ Cult 1: 229–238.

    Article  CAS  Google Scholar 

  34. Ikemori YK (1987) Epicormic shoots from the branches of Eucalyptus grandis as an explant source for in vitro culture. Comm For Rev 66: 351–355.

    Google Scholar 

  35. Ito K, Doi K, Tatemichi Y, Shibata M (1990) Plant rejuvenation from protoplasts of Eucalyptus. Proc VIIth Intern Congress Plant Tissue and Cell Culture Amsterdam June 24–29 1990 Abstract Al-65.

    Google Scholar 

  36. Kawazu T, Doi K, Ohta T, Shinohara Y, Ito K, Shibata M (1990) Transformation of Eucalyptus (Eucalyptus saligna) using electroporation. Proc VIIth Intern Congress Plant Tissue and Cell Culture Amsterdam June 24–29 1990 Abstract A2–80.

    Google Scholar 

  37. Khuspe SS, Gupta PK, Kulkarni DK, Mehta V, Mascarenhas AF (1987) Increased biomass production by Eucalyptus. Can J For Res 17: 1361–1363.

    Article  Google Scholar 

  38. Kozai T (1991) Micropropagation under photoautotrophic conditions. In: de Bergh P, Zimmerman R (Eds.) Micropropagation Technologies and Application, Springer Science+Business Media New York, Dordrecht.

    Google Scholar 

  39. Lakshmi Sita G (1986) Progress towards clonal propagation of Eucalyptus grandis. In: Withers LA, Alderson PG (Eds.) Lakshmi Sita G, pp 159–166, Butterworths.

    Google Scholar 

  40. Lakshmi Sita G (1992) Micropropagation of Eucalyptus. In: Ahija MR (Ed.) Micropropagation of Woody Plants, pp 263–80, Kluwer, Dordrecht, the Netherlands.

    Google Scholar 

  41. Lakshmi Sita G, Sukanya C (1987) Improvement of forest trees by tissue culture. In: Reddy GM (Ed) Plant Cell And Tissue Culture of Economically Important Plants. Proc National Symposium pp 195–198.

    Google Scholar 

  42. Leborgne N, Dupou-Cezanne L, Teulieres C, Canut H, Tocanne JF, Boudet AM (1992) Lateral and rotational mobilities of lipids in specific cellular membranes of Eucalyptus gunnii cultivars exhibiting different freezing tolerance. Pl Physiol 100: 246–254.

    Article  CAS  Google Scholar 

  43. Leifert C, Waites WM (1990) Contaminants of plant tissue culture. International Assoc PI Tissue Culture Newsletter 60: 2–13.

    Google Scholar 

  44. Lloyd G, McCown B (1981) Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot tip culture. Comb Proc Int PI Prop Soc 30: 421–427.

    Google Scholar 

  45. MacRae S (1991) Agrobacterium mediated transformation of eucalypts to improve rooting ability. In: IUFRO Symposium on Intensive Forestry: The Role of Eucalypts, Durban South Africa Sept 1: 117–125.

    Google Scholar 

  46. MacRae S, van Staden J (1990) In vitro culture of Eucalyptus grandis effects of gelling agents on propagation. J Plant Physiol 137: 249–251.

    Article  Google Scholar 

  47. Mascarenhas AF, Itazara S, Potdar V, Kulkaini DK, Gupta PK (1982) Rapid clonal multiplication of mature forest trees through tissue culture. In: Fujiwara A (Ed) Plant Tissue Culture,pp 719–720, Japan.

    Google Scholar 

  48. Mazalewski RL, Hackett WP (1979) Cutting propagation of Eucalyptus ficifola using cytokinin induced basal trunk shoots. Comb Proc Inter Pl Prop Soc 29: 118–124.

    Google Scholar 

  49. McComb JA, Bennett IJ (1982) Vegetative propagation of Eucalyptus using tissue culture and its application to forest improvement in Western Australia. Proc 5th Int Cong Plant Tissue and Cell Culture. In: Fujiwara A (Ed.), Plant Tissue Culture, pp 721–2.

    Google Scholar 

  50. McComb JA, Bennett IJ (1986) Eucalyptus (Eucalyptus spp). In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry 1: Trees pp 340–362.

    Google Scholar 

  51. McComb JA, Newell C, Arthur N (1986) Micropropagation of Verticordia grandis and other Verticordia species. The Plant Propagator 32: 12–14.

    Google Scholar 

  52. McComb JA, Wroth M (1986) Vegetative propagation of Eucalyptus resinifera and E. maculata using coppice cuttings and micropropagation. Aust For 16: 231–242.

    Google Scholar 

  53. McComb JA, Bennett IJ, van der Moezel PG, Bell DT (1989) Biotechnology enhances utilisation of Australian woody species for pulp fuel and land rehabilitation. Aust J Biotech 34: 297–301.

    Google Scholar 

  54. McComb JA, Bennett IJ, Stukely M, Crane C (1990) Selection and propagation of jarrah for dieback resistance. Comb Proc Intern PI Prop Soc 40: 86–90.

    Google Scholar 

  55. McComb JA, Bennett IJ, Cahill (1992) Selection, propagation, field and laboratory testing of jarrah (Eucalyptus marginata) resistant to dieback (Phytophthora cinnamomi). IUFRO/ AFOCEL Conference on Mass Production Technology for Genetically Improved Fast Growing Forest Tree Species Bordeaux France Sept 1992 2: 451–452.

    Google Scholar 

  56. McCown DD, McCown BH (1987) North American hardwoods. In: Bonga J, Durzan D (Eds.) Cell and Tissue Culture in Forestry, pp 247–260, Martinus Nijhoff, Dordrecht.

    Chapter  Google Scholar 

  57. Mindali D, Vieira JD, Soares MM, Osti NM, Figueiredo DB, Ribeiro MC (1992) In vitro Eucalyptus species contaminating bacteria identification. In AFOCEL Mass Production Technology for Genetically Improved Fast Growing Forest Tree Species AFOCEL Bordeaux France Sept 14 1992 2: 245–253.

    Google Scholar 

  58. Muralidharan EM, Mascarenhas AF (1987) In vitro plantlet formation by organogenesis in Eucalyptus camaldulensis and by somatic embryogenesis in Eucalyptus citriodora. Plant Cell Rep 6: 256–259.

    Article  Google Scholar 

  59. Muralidharan EM, Gupta PK, Mascarenhas AF (1989) Plantlet production through high frequency somatic embryogenesis in long term cultures of Eucalyptus citriodora. Plant Cell Reports 8: 41–43.

    Article  Google Scholar 

  60. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 18: 473–497.

    Article  Google Scholar 

  61. Ossor A, Boxus P (1992) Des aggolomérats de domes méristématiques: une nouvelle alternative dans la multiplication clonale d’Eucalpytus camaldulensis. In: AFOCEL Mass Production Technology for Genetically Improved Fast Growing Forest Tree Species, AFOCEL Bordeaux France Sept 14 2: 203–211.

    Google Scholar 

  62. Ouyang Q, Peng HZ, Li QQ (1981) Studies on the development of embryoids from Eucalyptus callus. Sci Silvae Sin 17: 1–7 (in Chinese).

    Google Scholar 

  63. Paton DM, Willings RR, Nicolls W, Pryor LD (1970) Rooting of stem cuttings of Eucalyptus; a rooting inhibitor in adult tissue. Aust J Bot 18: 175–183.

    Article  Google Scholar 

  64. Paton DM, Willings RR, Pryor LD (1981) Root-shoot gradients in Eucalyptus ontogeny. Ann Bot 47: 835–8.

    Google Scholar 

  65. Penchel RM, Kirby EG (1990) Establishment of cell culture systems for selected superior hybrids of Eucalyptus grandis hybrids. VIIth Intern Congress Plant Tissue and Cell Culture Amsterdam 199 Abstract Al-114.

    Google Scholar 

  66. Potter R, Jones MKG (1991) An assessment of genetic stability of potato in vitro by molecular and phenotypic analysis. PI Sci 76: 239–248.

    CAS  Google Scholar 

  67. Qin Chang-li, Kirby EG (1992) In vitro responses of cultured explants of Eucalyptus spp. In: AFOCEL Mass Production Technology for Genetically Improved Fast Growing Forest Tree Species AFOCEL Bordeaux France Sept 14 1992 2: 213–218.

    Google Scholar 

  68. Rao KS, Venkateswara R (1985) Tissue culture of forest trees clonal multiplication of Eucalyptus grandis. Plant Sci (Shannon) 40: 51–56.

    Article  Google Scholar 

  69. Rossetto M, Dixon KW, Bunne E (1992) Aeration, a simple method to control vitrification and improve in vitro culture of rare Australian plants. In vitro Cell Div. Biol. Plant 28P: 192–196.

    Google Scholar 

  70. Roux JJ le, Staden J van (1991) Micropropagation and tissue culture of Eucalyptus - a review. Tree Physiology 9: 435–477.

    Article  PubMed  Google Scholar 

  71. Salmon AD, (1990) Clonal propagation of the red flowering gum. Australian Horticulture 88: 63–68.

    Google Scholar 

  72. Sussex IM (1965) The origin and morphogenesis of Eucalyptus cell populations. In: White P, Groves A (Eds.) Proc Int Conf Pl Tissue Cult,pp 383–391, Berkley California.

    Google Scholar 

  73. Teulieres C, Boudet AM (1991) Isolation of protoplasts from different Eucalyptus species and preliminary studies on regeneration. Plant Cell Tissue and Organ Culture 25: 133–140.

    Google Scholar 

  74. Teulieres C, Alibert G, Marien JN, Boudet AM (1989a) Isolation and frost resistance screening of protoplasts from different clones of Eucalyptus. J Plant Physiol 134: 316–319.

    Article  Google Scholar 

  75. Teulieres C, Feuillet C, Boudet AM (1989b) Differential characteristics of cell suspension cultures initiated from Eucalyptus gunnii clones differing by their frost tolerance. Pl Cell Rep 8: 407–410.

    Article  CAS  Google Scholar 

  76. Teulieres C, Grima-Pettenati J, Curie C, Teissie J, Boudet AM (1991) Transient foreign gene expression in polyethylene/glucose treated or electropulsated Eucalyptus gunnii protoplasts. PI Cell Tissue and Organ Culture 25: 125–132.

    Google Scholar 

  77. Texier F, Faucher M (1986) Culture in vitro d’apex d’eucalyptus age Eucalyptus parvifolia Camb Annales de Recherches Sylvicoles, AFOCEL France 1985 pp 7–23.

    Google Scholar 

  78. Trindade IT, Ferriera JG, Paris MS, Aloni R (1990) The role of cytokinin and auxin in rapid multiplication of shoots of Eucalyptus globulus grown in vitro. Australian Forestry 53: 221–223.

    Article  Google Scholar 

  79. Van der Moezel PG, Bell DT, Bennett IJ, Strawbridge M, McComb JA (1990) The development of salt tolerant clonal trees in Australia. Comb Proc Intern Pl Prop Soc 40: 73–75.

    Google Scholar 

  80. Venketeswaran S, Gandhi V (1980) Protoplast isolation and culture from tree genera. In vitro 16: 232.

    Google Scholar 

  81. Warrag EI, Lesney MS, Rockwood DL (1990) Micropropagation of field tested superior Eucalyptus grandis hybrids. New For 4: 67–80.

    Article  Google Scholar 

  82. Warrag EI, Lesney MS, Rockwood DL (1991) Nodule culture and regeneration of Eucalyptus grandis hybrids. Pl Cell Rep 9: 586–589.

    Article  Google Scholar 

  83. Willyams D, Whiteman P, Cameron J, Chandler SF (1992) Inter-and intra-family variability of rooting capacity in micropropagated Eucalyptus globulus and Eucalyptus nitens. In: AFOCEL Mass Production Technology for Genetically Improved Fast Growing Forest Tree Species, AFOCEL Bordeaux France Sept 14 2: 177–181.

    Google Scholar 

  84. Wilson PJ (1992) The development of new clones of Eucalyptus globulus and E. globulus hybrids by stem cuttings propagation. In: AFOCEL Mass Production Technology for Genetically Improved Fast Growing Forest Tree Species, AFOCEL Bordeaux France Sept 14 1: 379–386.

    Google Scholar 

  85. Young PM, Hutchins AS, Canfield ML (1984) Use of antibiotics to control bacteria in shoot cultures of woody plants. PI Sci Letts 34: 203–9.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Biological and Environmental Sciences, Murdoch University, Perth, Western Australia, 6150, Australia

    J. A. McComb

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  1. Department of Biological Sciences, University of Durham, Leicester, UK

    K. Lindsey

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McComb, J.A. (1995). Clonal propagation of eucalypts. In: Lindsey, K. (eds) Plant Tissue Culture Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0103-2_32

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  • DOI: https://doi.org/10.1007/978-94-009-0103-2_32

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