The initiation and maintenance of plant cell suspension cultures
Ideally, a plant cell suspension culture should consist of a population of single cells suspended, through continuous agitation, in a liquid nutrient medium. However, in only the rarest of instances, if ever, does such a culture exist. Increased cell dissociation means increased culture uniformity and consequently, most researchers strive to achieve as fine a cell suspension as possible. However, some degree of cell aggregation generally has to be tolerated and so-called ‘fine’ suspension cultures consist of micro- to submacroscopic colonies made up of ca. 5–200 cells. Furthermore, cultures consisting of larger aggregates (e.g. 0.5–1.0 mm in diameter) usually are more readily attainable, grow perfectly well and depending on the aim of the research are often sufficient to meet all requirements. Indeed, in certain instances a degree of cell aggregation has been argued to be greatly beneficial — e.g. in retaining the totipotent character of gramineous suspension cultures [1, 2] and in obtaining enhanced yields of desirable secondary metabolites from in vitro systems .
KeywordsSuspension Culture Pack Cell Volume Friable Callus Catharanthus Roseus Chromium Trioxide
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