In Vitro Effect of Glucose on Intralymphocyte Free Magnesium
It has been known from some time that diabetes mellitus and the plurimetabolic syndrome are characterized by a decrease of intracellular magnesium [1,2]. Despite the in vivo important role of magnesium on glucose metabolism, very little is known on the mechanisms linking glucose and magnesium intracellular homeostasis. It has been reported that in vitro incubation of non-nucleated cells (erythrocytes) in the presence of glucose was followed by a decrease of ionized magnesium as measured by a NMR technique . The recent synthesis of a new fluorescent magnesium-sensitive dye has allowed the measurement of intracellular free magnesium in nucleated cells. We therefore measured lymphocythe free magnesium in nucleated cells, i.e. human lymphocytes, incubated in the presence or in the absence of glucose.
KeywordsNucleate Cell Glucose Effect Recent Synthesis Vitro Effect Intracellular Magnesium
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