Isolation and Identification of Arbuscular Mycorrhizal Fungi (AMF)
Arbuscular mycorrhizal fungi (AMF) in the order Glomales colonize the root systems of the large majority of land plant species. They often have beneficial influence on growth and survival of their host due to better mineral nutrition and increased tolerance in abiotic and biotic stress situations. However, the functional and taxonomic characterization of individual AMF isolates is limited because of their obligate biotrophy. Morphological characteristics of the relatively large, asexual spores have been used for the identification and taxonomy of glomalean fungi. Difficulties in the identification of spores arise from the frequently poor state of spores isolated from the field and the few morphological characters available to discriminate species. Recent advances in biochemical and molecular techniques provide a promising tool to overcome these problems. In this study, AMF from different sites were characterised according to their morphological features as well as using isozymes as molecular markers.
KeywordsInternal Transcribe Spacer Arbuscular Mycorrhizal Fungus Malate Dehydrogenase Arbuscular Mycorrhiza Arbuscular Mycorrhizal Fungus Species
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