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Quantification of Phytophthora Infestans During Early Stages of Pathogenesis

  • R. Stierl
  • Ulrike Steiner
  • H.-W. Dehne
Part of the Developments in Plant Pathology book series (DIPP, volume 11)

Abstract

Phytophthora infestans is the pathogen causing the highly destructive disease late blight affecting both tomato and potato. The fungus attacks all aboveground parts of the plants and additionally in potato the tubers. Leaf lesions first appear as water-soaked spots, which may enlarge rapidly into brown necrotic lesions cover large areas of the leaves. Later, the fungus may be observed occasionally as mouldy growth around the undersides of large brown lesions. Therefore, the assessment of disease severity is usually done indirectly by the estimation of the amount of damaged area of the plant leaves. In research, i. e. breeding of resistant cultivars or development of new fungicides, techniques are essential which allow the evaluation of the development of P. infestans before any damage is visible. Microscopical and serological detection methods for a direct quantification of P. infestans in the leaf tissue, also during the early stages of pathogenesis, are presented.

Keywords

Fungal Biomass Germ Tube Resistant Cultivar ELISA Test Phytophthora Infestans 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Beckman, K.B., Harrison, J.G. and Ingram, D.S. (1994) Optimization of a polyclonal enzyme-linked immunosorbent assay (ELISA) of fungal biomass for use in studies of plant defence responses. Physiol. Mol. Pl. Path. 44, 19–32.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • R. Stierl
    • 1
  • Ulrike Steiner
    • 1
  • H.-W. Dehne
    • 1
  1. 1.Insitut für PflanzenkrankheitenRheinische Friedrich-Wilhelms Universität BonnBonnGermany

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