Isolation of Micromycetes from Pea Seeds

  • E. Prokinová
Part of the Developments in Plant Pathology book series (DIPP, volume 11)


Seed’s quality is one of the basic assumption for healthy growth. Next to other factors it is determined by health status of the seed. On the seed come up multitude of pathogens as well as saprophytic micromycetes (Neergaard 1979, Richardson 1979). For investigation of their influence upon seeds, mutual relations and their impact upon health condition of seeds and final recommendation for seed practise exact diagnostics of seed-borne fungi is necessary. Very accurate methods were elaborated recently for some important phytopathogenic fungi (Hansen and Wick 1993, Fox 1993, Okrouhlá 1994) and their research and development go forward very quickly. But these methods are relatively demanding upon laboratory equipment and their use in current practice is not substantial (except some exceptions - e.g. rapid disease kids for Phytophthora spp., Pythium spp., Rhizoctonia spp.). Here it is good to perceive the difference between detection and diagnosis - these denominations are interchanged even in professional community and many of detection methods are presented as diagnostic methods. It is actually well-known that mere presence of pathogen is not enough for objective evaluation of origin and development of the disease. Therefore most widespread remains classical detection methods, in fungi case isolation on agar plates and following determination of isolates according morphological or biochemical characteristics. The isolation method of pathogens on agar plates gives also partial information about relations between present microscopic fungi and plant.


Aspergillus Niger Botrytis Cinerea Trichoderma Harzianum Alternaria Alternata Cladosporium Cladosporioides 
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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • E. Prokinová
    • 1
  1. 1.Department of Plant ProtectionCzech Agriculture UniversityPrague 6-SuchdolCzech Republic

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