Isolation of Micromycetes from Pea Seeds
Seed’s quality is one of the basic assumption for healthy growth. Next to other factors it is determined by health status of the seed. On the seed come up multitude of pathogens as well as saprophytic micromycetes (Neergaard 1979, Richardson 1979). For investigation of their influence upon seeds, mutual relations and their impact upon health condition of seeds and final recommendation for seed practise exact diagnostics of seed-borne fungi is necessary. Very accurate methods were elaborated recently for some important phytopathogenic fungi (Hansen and Wick 1993, Fox 1993, Okrouhlá 1994) and their research and development go forward very quickly. But these methods are relatively demanding upon laboratory equipment and their use in current practice is not substantial (except some exceptions - e.g. rapid disease kids for Phytophthora spp., Pythium spp., Rhizoctonia spp.). Here it is good to perceive the difference between detection and diagnosis - these denominations are interchanged even in professional community and many of detection methods are presented as diagnostic methods. It is actually well-known that mere presence of pathogen is not enough for objective evaluation of origin and development of the disease. Therefore most widespread remains classical detection methods, in fungi case isolation on agar plates and following determination of isolates according morphological or biochemical characteristics. The isolation method of pathogens on agar plates gives also partial information about relations between present microscopic fungi and plant.
KeywordsAspergillus Niger Botrytis Cinerea Trichoderma Harzianum Alternaria Alternata Cladosporium Cladosporioides
Unable to display preview. Download preview PDF.
- Czyÿewska S.(1976) Studies on Fungi Infesting Seeds of Pea (Pisum sativum L).Bull. warzywniczy XIX, IV. Ochrona roœlin: 271–288Google Scholar
- Fox R. T. V. (1993)Principles og Diagnostic Techniques in Plant Pathology. International Mycological Institute, UK Press, Cambridge.Google Scholar
- 3.Hansen M. A. and R. L. Wick, (1993) Plant Disease Diagnosis: Present Status and Future Prospects. In:Andrew J. H. and I. C. Tommerup (eds.) (1993)Advances in Plant Pathology, vol. 10: 65 - 126Google Scholar
- 4.Neergaard P.(1979) Seed Pathology, Academic PressGoogle Scholar
- 5.Okrouhlá M., (1994)Rychlá diagnostika chorob rostlin. Studijni informace, Rostlinná výrobaNo.3. UZPI PrahaGoogle Scholar
- 6.Richardson M. J. (ed.) (1979) An Annotated List of Seed-Borne Pathogens. Phytopathological Papers, no 23, ISTA Seed health Testing Handbook1.1:186–192Google Scholar