PCR-Based Diagnostics of Agricultural Pests and Diseases

An Introduction to the Basic with Examples
  • Jürg E. Frey
  • Beatrice Frey
Part of the Developments in Plant Pathology book series (DIPP, volume 11)


Since its introduction about a decade ago, the polymerase chain reaction (PCR) has become a widely accepted technique in many agricultural research institutions. By now, a staggering number of variations to the basic PCR amplification process has been described, and even an approximate overview over these would be beyond the scope of this contribution (see Newton and Graham [1994] for a comprehensive review). Rather, we give a short introduction to the principles of PCR and exemplify the benefit of basic methods for agricultural diagnostics.


Polymerase Chain Reaction Molecular Diagnostics Scale Insect Pheromone Trap Thrips Species 
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  1. Frey, J.E. and Frey, B. (1995) Molecular identification of six species of scale insects (Quadraspidiotus sp.) by RAPD-PCR: assessing the field-specificity of pheromone traps. Molecular Ecology 4, 777–780.CrossRefGoogle Scholar
  2. Hilber, U.W., Hilber-Bodmer, M., Schiiepp, H., and Frey, J.E. (1997) Diagnosis and identification of pathogens causing apple storage rots, by using PCR-RFLP and allele-specific amplification, (this issue).Google Scholar
  3. Newton, C.R. and Graham, A. (1994) PCR, BIOS Scientific Publishers, Oxford. (German translation: PCR, Spektrum Akademischer Verlag, Heidelberg)Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Jürg E. Frey
    • 1
  • Beatrice Frey
    • 1
  1. 1.Swiss Federal Research Station for Fruit-Growing, Viticulture and HorticultureWädenswilSwitzerland

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