Molecular Diagnosis of Toxigenic Fusarium Species

  • Giuseppina Mule’
  • Antonio Logrieco
Part of the Developments in Plant Pathology book series (DIPP, volume 11)


Correct identification of species in Fusarium genera is important because many species are plant pathogens and same isolates produce potent mycotoxins (Marasas et al., 1984). Methods that have been used in Fusarium systematics include DNA-DNA hybridization (Ellis, 1988), RFLP analysis (Manicom & Baayen, 1993), RAPD markers (Schilling et al., 1996), isozyme (Huss et al, 1993), sequence analysis (O’Donnell, 1996) and electrophoretic karyotyping (Fekete et al., 1993). DNA-DNA hybridization may reflect relatedness at the level of isolates, but RFLP and karyotype analyses typically differentiate at level of genetically isolated groups. Problem with F. oxysporum in Boehm et al. (1994) where they found karyotype variation in strains belonging to the same VCG. RAPDs have been used to detect genetic differences between and within species. Variation in nucleotide sequence demonstrates relatedness at different levels depending on the sequence chosen for analysis. O’Donnell (1996) recently have developed a DNA sequence database using four loci: beta tubulin gene introns/exons, mitochondrial small subunit (mtSSU) rDNA, nuclear large subunit (LSU) rDNA, and nuclear rDNA internal transcribed spacer (ITS) region. We found positive correlation between phylogeny, secondary metabolites production and the sequence of nuclear LSU 28S rDNA.


Internal Transcribe Spacer Secondary Metabolite Production Trichothecene Mycotoxin Fusarium Strain Electrophoretic Karyotype 
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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Giuseppina Mule’
    • 1
  • Antonio Logrieco
    • 1
  1. 1.Istituto Tossine e micotossine da parassiti vegetali, C.N.RItaly

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