Abstract
Recently we have succeeded to isolate a new nepo-type virus from a reverted blackcurrant. We have developed an immunocapture-reverse transcriptase-PCR (IC-RT-PCR) method to detect the virus, and have demonstrated that the virus is associated both with the E and R forms of reversion disease originating from Scandinavia, Scotland and New Zealand. Due to its origin and its consistent association with the different forms of reversion, but not with any other virus diseases of Rites tested, we refer to the virus as Blackcurrant reversion associated virus (BRAV).
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References
Adams, A. N., and Thresh, J. M. 1987. Reversion of blackcurrant, in: R. H. Converse, (ed.), Virus Diseases of Small Fruits. USDA Agriculture Handbook No. 631. pp. 133–136
Erlich, H. A., Gelfand, D., and Sninsky, J. J. 1991. Recent advances in the polymerase chain reaction. Science 252, 1643–1651
Jones, A. T. 1992. Appendix I. Recommended indexing procedures for detecting viruses, viroids, mycoplasma- and rickettsia-like organisms and virus-like diseases in small fruit crops: Currants and Gooseberries (Ribes). Acta Hortic. 308, 51–154
Jones, A. T. 1995. Recent developments affecting the control of two important diseases of small fruit crops in the UK. Acta Hortic. 385, 64–69
Lemmetty, A., Latvala S., Jones, A.T., McGavin, W.J., Cox, S., Susi, P.E., and Lehto, K. 1996. Purification and properties of a new virus from blackcurrant, its affinities with nepoviruses and its close association with blackcurrant reversion disease. Phytopathology (accepted for publication)
Nolasco, G. de Bias, C., Torres, V., and Ponz, F. 1993. A method combining immunocapture and PCR amplification in a microtitre plate for detection of plant viruses and subviral pathogens. J. Virol Methods 45, 201–218
Wetzel, T., Candresse, T., Ravelondandro, M., and Dunez, J. 1991. A polymerase chain reaction assay adapted to plum pox potyvirus detection. J. Virol Methods 33, 355–365
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Lehto, K., Latvala, S., Jones, A.T., Cox, S., Susi, P.E., Lemmetty, A. (1997). IC-RT-PCR Detection of Blackcurrant Reversion Associated Nepovirus, and Perspectives of Its Use for Diagnosis of the Reversion Disease. In: Dehne, HW., Adam, G., Diekmann, M., Frahm, J., Mauler-Machnik, A., van Halteren, P. (eds) Diagnosis and Identification of Plant Pathogens. Developments in Plant Pathology, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0043-1_36
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DOI: https://doi.org/10.1007/978-94-009-0043-1_36
Publisher Name: Springer, Dordrecht
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