Development of a Rapid Method for The Detection and Identification of Colletotrichum Acutatum

  • K. J. D. Hughes
  • C. R. Lane
  • R. T. A. Cook
Part of the Developments in Plant Pathology book series (DIPP, volume 11)

Abstract

The Central Science Laboratory has developed a rapid, easy to use “bio-amplification ELISA” method for the detection and identification of quiescent Colletotrichum acutatum infection in strawberry plant material. We believe this is the first use of an ELISA system for the routine detection of an EC quarantine fungus. The detection system has been extensively used on imported and UK derived material and is currently under evaluation in other EC laboratories.

Keywords

Spore Suspension Plant Health Serological Assay Standard ELISA Routine Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barker, I., Brewer, G., Cook, R.T.A, Crossley, S. and Freeman S. (1994) Strawberry black spot disease (Colletotrichum acutatum). In Modern assays for plant pathogenic fungi: Identification, detection and Quantification, (eds. A. Schots, F.M. Dewey and R.P. Oliver), pp 179–182. CABI, Oxford UK.Google Scholar
  2. Cook, R.T.A. (1993) Strawberry black spot caused by Colletotrichum acutatum. In Plant health and the European Single Market. BCPC monograph no 54 (ed D. Ebbles), pp 301–304. BCPC, Famham, UK.Google Scholar
  3. Cook, R.T.A., Barker I., Brewer, G., Crossley, S., Freeman, S. & Lane, C. R. (1995). Detection of Strawberry black spot (Colletotrichum acutatum) with monoclonal antibodies. OEPP/EPPO 25 pp 57–63.Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • K. J. D. Hughes
    • 1
  • C. R. Lane
    • 1
  • R. T. A. Cook
    • 1
  1. 1.Central Science LaboratoryMAFFYorkUK

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