Abstract
In Germany, one of the most important crop rotation diseases of oilseed rape is caused by the fungus Verticillium dahliae (Krüger, 1989; Amelung et al., 1996). The pathogen persists in soil for many years with microsclerotia (MS). The inoculum density of V. dahliae plays a critical role in disease development of oilseed rape. A chemical treatment of the pathogen, like soil fumigation, is not allowed in Germany. Therefore, our aim was to develop a disease prognosis for oilseed rape on the basis of the MS concentration in soil. Various methods, e.g. direct soil assays or bioassays, have been developed for detection and quantification of the inoculum in soil (Evans et al., 1974; Harris et al., 1993). These classical techniques, however, are time consuming and require mycological expertise. An immunoassay for the assessment of V. dahliae in soil can be considered as a faster and easier detection method.
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© 1997 Kluwer Academic Publishers
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Heppner, C., Heitefuss, R. (1997). Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Verticillium Dahliae Detection in Soil. In: Dehne, HW., Adam, G., Diekmann, M., Frahm, J., Mauler-Machnik, A., van Halteren, P. (eds) Diagnosis and Identification of Plant Pathogens. Developments in Plant Pathology, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0043-1_22
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DOI: https://doi.org/10.1007/978-94-009-0043-1_22
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