Monoclonal Antibody-Based Assays for Detection and Quantification of Botrytis Cinerea

  • Frances M. Dewey
  • Louise Cole
Part of the Developments in Plant Pathology book series (DIPP, volume 11)

Abstract

Botrytis cinerea Pers is responsible for the widespread grey mould disease of plants throughout temperate climates. It can infect an amazing diverse variety of plants but it is most noted for the serious losses that it causes in the fresh fruit, vegetable and cut flower industries The air-borne conidia germinating on plant surfaces either cause an immediate damaging, necrotrophic rot in growing plants or, they remain localized in the outer tissues until harvest and shipment where upon the organism then becomes invasive causing considerable post harvest losses. Although losses can be minimised by repeated applications of fungicides throughout the growing season there are increasing environmental and economic concerns about the prophylactic use of fungicides particularly with respect to fresh fruits and vegetables where fungicide residues have been detected. In order to target fungicide applications to the periods of highest risk and thereby reduce the number of spray applications during the growing season, we need to be able to measure not only the environmental factors that have the greatest effect on the spread of the conidia and development of the disease but also quantify the numbers of air-borne spores present at high risk times so that disease forecasting models can be made more accurate. Information on levels of air-borne conidia would also be invaluable in determining the growth stage and route by which quiescent infections are initiated. Furthermore, in order to minimize post harvest losses we also need to be able to detect symptomless infections before shipment of produce. We have addressed these various needs by developing immunological assays using the Botrytis - monoclonal antibody BC-KH4 raised by Bossi and Dewey (1992).

Keywords

Fresh Fruit Botrytis Cinerea Grape Skin Post Harvest Loss Digital Image Analysis System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bossi, R. and Dewey, F.M. (1992). Development of a monoclonal antibody-based immunodetection assay for Botrytis cinerea. Plant Pathology 41, 472–482.CrossRefGoogle Scholar
  2. Bossi, R., Cole, L., Spier, A.D. and Dewey, F.M. (1994). Monoclonal antibody-based ELISA for detection of mycelial antigens of Botrytis cinerea in fruits and vegetables. In: Modern assays for Detection of Plant Pathogenic Fungi: Identification, Detection and Quantification (ed. A. Schots, F.M.Dewey k R. Oliver), pp.165–172. CAB International: Wallingford, UKGoogle Scholar
  3. Cole, L. Immunocytochemical studies of fungal cell surface molecules and the Vicia faba. Botrytis interaction. Ph.D. Thesis, Oxford Brookes University, 1996, pp. 200Google Scholar
  4. Cole, L., Dewey, F. M. and Hawes, C. R. (1996). Infection mechanisms of Botrytis species: prepenetration and preinfection processes of dry and wet conidia. Mycological Research, 100. 277–282.CrossRefGoogle Scholar
  5. Dewey, F.M. (1996) Production and use of monoclonal antibodies for the detection of fungi. In: Diagnostics in Crop Production, pp 85–93, BCPC Symposium 65, ed G. MarshallGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Frances M. Dewey
    • 1
  • Louise Cole
    • 2
  1. 1.Department of Plant SciencesUniversity of OxfordOxfordUK
  2. 2.School af Biological SciencesOxford Brookes UniversityOxfordUK

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