Testing Viability and Germination of Plasmodiophora Brassicae (Wor.) Resting Spores Using the Fluorescence Microscope Technique
The usefulness of the fluorescence microscope method for the assessment of resting spore activity of Plasmodiophora brassicae (Wor.) was assessed. The influence of root exudates of cruciferous and non-cruciferous plants on the germination of P. brassicae resting spores was investigated with a view to the future application for the biocontrol of clubroot.
The stained spores were classed in correspondence with the categories 1–7 of DOYLE (1990, unpublished): Non-viable spores when classed as germinated or dead ones, showed a blue sporewall and red fluorescence on the inside, while viable spores showed the blue sporewall alone.
The influence of root exudates from cruciferous and non-cruciferous plants on the germination of P. brassicae resting spores is described by the percentage of viable, non-germinated spores.
KeywordsRoot Exudate Spore Germination Staining Reaction Biotrophic Fungus Plasmodiophora Brassicae
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