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Testing Viability and Germination of Plasmodiophora Brassicae (Wor.) Resting Spores Using the Fluorescence Microscope Technique

  • Katrain Vent-Schmidt
  • H. Bochow
Part of the Developments in Plant Pathology book series (DIPP, volume 11)

Abstract

The usefulness of the fluorescence microscope method for the assessment of resting spore activity of Plasmodiophora brassicae (Wor.) was assessed. The influence of root exudates of cruciferous and non-cruciferous plants on the germination of P. brassicae resting spores was investigated with a view to the future application for the biocontrol of clubroot.

The stained spores were classed in correspondence with the categories 1–7 of DOYLE (1990, unpublished): Non-viable spores when classed as germinated or dead ones, showed a blue sporewall and red fluorescence on the inside, while viable spores showed the blue sporewall alone.

The influence of root exudates from cruciferous and non-cruciferous plants on the germination of P. brassicae resting spores is described by the percentage of viable, non-germinated spores.

Keywords

Root Exudate Spore Germination Staining Reaction Biotrophic Fungus Plasmodiophora Brassicae 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Katrain Vent-Schmidt
    • 1
  • H. Bochow
    • 1
  1. 1.Agric.-Hortic. Fac., Div. PhytomedicineHumboldt-University BerlinBerlinGermany

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