Advertisement

Isolation of DNA from Preserved Specimens

  • De-Xing Zhang
  • Godfrey M. Hewitt
Chapter

Abstract

Fresh tissue/blood samples provide the best sources of DNA for biological analyses, but their availability is often limited. Although cryopreservation (freezing) has proved to be the most effective method for preserving various tissues, the most routine and popular ways for preserving animal samples are to use alcohol, formalin or buffered solutions as preservatives. To study population biology, systematics and biodiversity at the molecular level, it may be of great interest in many cases to have some historical knowledge about the species or populations in question; and researchers sometimes need to re-examine previous studies. Furthermore, sample collection for many species, or populations, may not be possible at a given time because of difficulties and costs due to remoteness, population decline or even extinction.

Keywords

Preserve Specimen Full Speed Styrene Divinylbenzene Isopropanol Precipitation Chelex Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Walsh PS, Metzger DA, Higuchi R (1991). Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10: 507–513Google Scholar
  2. 2.
    Greer CE, Wheeler CM, Manos MM (1994). Sample preparation and PCR amplification from paraffin-embedded tissues. PCR Methods and Applications 4: s113–s122Google Scholar
  3. 3.
    Tan AM, Orrego C (1992). DNA stabilization and amplification from museum collections of extracts originally intended for allozyme analysis. Molecular Ecology 1: 195–197CrossRefGoogle Scholar
  4. 4.
    Pääbo S (1990). ‘Amplifying ancient DNA’ In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, pp 159–166Google Scholar
  5. 5.
    Hagelberg E, Clegg JB (1991). Isolation and characterization of DNA from archaeological bone. Proceedings of the Royal Society of London, B 244: 45–50CrossRefGoogle Scholar
  6. 6.
    Poinar HN, Poinar GO, Cano RJ (1992). Extracting DNA from amber embedded organisms. Ancient DNA Newsletter 1 (2): 26–27Google Scholar
  7. 7.
    Higuchi R, vonBeroldigen CH, Sensabaugh GF, Erlich HA (1988) DNA typing from single hairs. Nature 332: 543–546CrossRefGoogle Scholar
  8. 8.
    Wilson MR, Polanskey D, Butler J, DiZinno JA, Replogle J, Budowle B (1995). Extraction, PCR amplification and sequencing of mitochondrial DNA from human hair shafts. BioTechniques 18: 662–669Google Scholar
  9. 9.
    Höss M, Kohn M, Pääbo S, Knauer F, Schröder W (1992) Excrement analysis by PCR. Nature 359: 199CrossRefGoogle Scholar
  10. 10.
    Hänni C, Brousseau T, Laudet V, Stehelin D (1995). Isopropanol precipitation removes PCR inhibitors from ancient bone extracts. Nucleic Acids Research 23: 881–882CrossRefGoogle Scholar
  11. 11.
    Dubeau L, Chandler LA, Gralow JR, Nichols PW, Jones PA (1986). Southern blot analysis of DNA extracted from formalin-fixed pathology specimens. Cancer Research 46: 2964–2969Google Scholar
  12. 12.
    Jackson DP, Hayden JD, Quirke P (1991) ‘Extraction of nucleic acid from fresh and archival material’ In: McPherson MJ, Quirke P, Taylor GR (eds) PCR: A Practical Approach. IRL Press at Oxford University Press, Oxford, pp29–50Google Scholar
  13. 13.
    Santos AC, Osorio-Almeida L (1993). Simultaneous extraction of RNA and DNA from paraffin-embedded tissues. Trends in Genetics 9:231CrossRefGoogle Scholar
  14. 14.
    Premoli-de-Percoco G, Guevara P, Galindo I, Ramirez JL (1994). Rapid recovery of DNA from paraffin-embedded tissue sections for routine Polymerase Chain Reaction analysis. Methods in Molecular and Cellular Biology 4: 266–268.Google Scholar

Copyright information

© Chapman & Hall 1998

Authors and Affiliations

  • De-Xing Zhang
  • Godfrey M. Hewitt

There are no affiliations available

Personalised recommendations