Special DNA Extraction Methods for Some Animal Species

  • De-Xing Zhang
  • Godfrey M. Hewitt
Chapter

Abstract

In some organisms it is difficult to obtain high quality DNA due to factors such as pronounced nuclease activity or the presence of polyphenols and polysaccharides. Technically, two factors could further affect the quality of DNA prepared from such organisms: the preservation method used for sample conservation and the protocol employed for DNA extraction. Sometimes, however, the poor quality of DNA is simply due to other factors such as food or faeces remaining inside the abdomen of insects or the stomach of higher animals, which could promote the degradation of DNA and contribute to contamination and enzymatic inhibition of PCR amplification or restriction enzyme digestion. These body parts are better removed before DNA extraction, where possible. More information on sample preservation is given in Box 3.1.

Keywords

Phenol Extraction Isoamyl Alcohol Clean Tube Guanidinium Thiocyanate Autoclave Distil Water 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Towner P (1991). ‘Purification of DNA’. In: Brown TA (ed) Essential Molecular Biology: A Practical Approach (Volume 1). IRL Press at Oxford University Press, Oxford, pp. 47–68.Google Scholar
  2. 2.
    Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299.CrossRefGoogle Scholar
  3. 3.
    Laulier M, Pradier E, Bigot Y, Periquet G (1995) An easy method for preserving nucleic acids in field samples for later molecular and genetic studies without refrigerating. Journal of Evolutionary Biology 8: 657–663.CrossRefGoogle Scholar

Copyright information

© Chapman & Hall 1998

Authors and Affiliations

  • De-Xing Zhang
  • Godfrey M. Hewitt

There are no affiliations available

Personalised recommendations