Special DNA Extraction Methods for Some Animal Species
In some organisms it is difficult to obtain high quality DNA due to factors such as pronounced nuclease activity or the presence of polyphenols and polysaccharides. Technically, two factors could further affect the quality of DNA prepared from such organisms: the preservation method used for sample conservation and the protocol employed for DNA extraction. Sometimes, however, the poor quality of DNA is simply due to other factors such as food or faeces remaining inside the abdomen of insects or the stomach of higher animals, which could promote the degradation of DNA and contribute to contamination and enzymatic inhibition of PCR amplification or restriction enzyme digestion. These body parts are better removed before DNA extraction, where possible. More information on sample preservation is given in Box 3.1.
KeywordsPhenol Extraction Isoamyl Alcohol Clean Tube Guanidinium Thiocyanate Autoclave Distil Water
Unable to display preview. Download preview PDF.
- 1.Towner P (1991). ‘Purification of DNA’. In: Brown TA (ed) Essential Molecular Biology: A Practical Approach (Volume 1). IRL Press at Oxford University Press, Oxford, pp. 47–68.Google Scholar