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Fluorescent Labelling of SSRS and Automated Detection

  • Michele Morgante
  • Antonella Pfeiffer
  • Irena Jurman
  • Gianpaolo Paglia
  • Angelo M. Olivieri
Chapter

Abstract

Fluorescent labelling of fragments is an alternative to radioactivity (32P or 33P) and the fragments can be separated with an automated sequencing machine. This allows very accurate sizing of the fragments, especially if a size standard is run together in the same lane with the sample to be sized.

Keywords

Automate Sequencer Size Standard Fluorescent Labelling Automate Detection Final Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Reference

  1. 1.
    Igloi, G.L. and Schiefermayr, E. (1993). Enzymatic addition of fluorescein-riboUTP to oligonucleotides results in primers suitable for DNA sequencing and PCR. BioTechniques 15: 486–497.Google Scholar

Copyright information

© Chapman & Hall 1998

Authors and Affiliations

  • Michele Morgante
  • Antonella Pfeiffer
  • Irena Jurman
  • Gianpaolo Paglia
  • Angelo M. Olivieri

There are no affiliations available

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