Fluorescent Labelling of SSRS and Automated Detection
Fluorescent labelling of fragments is an alternative to radioactivity (32P or 33P) and the fragments can be separated with an automated sequencing machine. This allows very accurate sizing of the fragments, especially if a size standard is run together in the same lane with the sample to be sized.
KeywordsAutomate Sequencer Size Standard Fluorescent Labelling Automate Detection Final Method
- 1.Igloi, G.L. and Schiefermayr, E. (1993). Enzymatic addition of fluorescein-riboUTP to oligonucleotides results in primers suitable for DNA sequencing and PCR. BioTechniques 15: 486–497.Google Scholar