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Automation and the Polymerase Chain Reaction

  • Richard G. Keightley
Chapter

Abstract

The complexity of set-up, the small volumes used, and the usual need to process many DNA samples simultaneously for the polymerase chain reaction (PCR) make this amplification process a prime candidate for automation. The potential benefits of using automation are:
  • Improved results by elimination of manual pipetting errors

  • Reduction of contamination with foreign DNA

  • Higher sample throughput

  • Time saving for the operator

Automation of DNA sample processing has been available for some time in the form of robotic pipetting workstations. These are capable of handling all the PCR reaction set-up steps prior to thermo-cycling. One such system is the Biomek Automated Workstation (Beckman Instruments, Fullerton CA, USA) which is in common use for PCR (1–3).

Keywords

Polymerase Chain Reaction High Sample Throughput MP20 MP200 Robotic Workstation Deepwell Plate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1998

Authors and Affiliations

  • Richard G. Keightley

There are no affiliations available

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