Single-Strand Conformation Polymorphism (SSCP) Analysis
Single-strand conformation polymorphism (SSCP) analysis (1, 2) is technically the most simple of the various methods available for rapid screening of DNA fragments for nucleotide sequence polymorphisms (3), although it has a sensitivity comparable to other methods and, with properly designed screening routines, can have a mutation detection efficiency of 100% (4). The technique relies on variation in electrophoretic mobility of secondary structures formed by single-stranded DNA (ssDNA) fragments of different primary structure (i.e. nucleotide sequence). DNA samples, usually PCR products, are denatured by heat and/or chemical denaturants, and electrophoresed into a non-denaturing gel.
KeywordsSilver Nitrate Solution Nucleic Acid Research SSCP Analysis Negative Control Reaction SSCP Pattern
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