RFLP Analysis

  • Reinhold Brettschneider


Restriction Fragment Length Polymorphism (RFLP) analysis is based on two techniques that are widely used in modern molecular biology: the restriction endonuclease digestion of DNA and the transfer of DNA fragments to a filter, onto which can then be hybridized a labelled DNA fragment (1). Type II restriction endonucleases of bacteria recognize and cut specific nucleotide motifs in a DNA sequence (the enzymes commonly used for RFLP analysis recognize 4–6 base-pair sequences). They are, therefore, capable of reducing complex DNA, such as plant DNA, to a population of fragments with discrete sizes. In practice, fragments range in size from a few to more than several thousand base-pairs.


Restriction Fragment Length Polymorphism High Specific Activity Restriction Fragment Length Polymorphism Analysis Restriction Fragment Length Polymorphism Marker Geiger Counter 
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© Chapman & Hall 1998

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  • Reinhold Brettschneider

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