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RFLP Analysis

  • Reinhold Brettschneider
Chapter

Abstract

Restriction Fragment Length Polymorphism (RFLP) analysis is based on two techniques that are widely used in modern molecular biology: the restriction endonuclease digestion of DNA and the transfer of DNA fragments to a filter, onto which can then be hybridized a labelled DNA fragment (1). Type II restriction endonucleases of bacteria recognize and cut specific nucleotide motifs in a DNA sequence (the enzymes commonly used for RFLP analysis recognize 4–6 base-pair sequences). They are, therefore, capable of reducing complex DNA, such as plant DNA, to a population of fragments with discrete sizes. In practice, fragments range in size from a few to more than several thousand base-pairs.

Keywords

Restriction Fragment Length Polymorphism High Specific Activity Restriction Fragment Length Polymorphism Analysis Restriction Fragment Length Polymorphism Marker Geiger Counter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Chapman & Hall 1998

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  • Reinhold Brettschneider

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