The Verification of a Newly Discovered Hepatitis B Virus Subtype Based on Sequence Analysis

  • Qingqing Yi
  • Lei Ma
  • Qinan Jia
  • Jianfeng He
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)


Hepatitis B virus (HBV) infection is one of the most serious global problems to human health. It has great importance to study HBV through the analysis of its phylogenetic tree. There has been previously reported that researchers had found a new HBV subtype in Xishuangbanna, Yunnan, China, named HBV/B6. In this paper, we propose a series of sequence analysis methods on a new data set that contains the reported one. During the analysis, several kinds of bioinformatics software are involved. The experimental results prove the existence of the newly found subtype HBV/B6 of Xishuangbanna in the reported literature. Moverover, the C gene of the newly found subtype contains a reorganization structure of HBV/B and HBV/C, which provides a positive reference for HBV derivation exploration. The conclusion represents certain significance on phylogenetic studies of hepatitis B virus.


Hepatitis B virus Subtype The X/PreC gene 


  1. 1.
    Goldstein ST, Zou F, Hadler SC et al (2005) A mathematical model to estimate global hepatitis B disease burden and vaccination impact. Int J Epidemiol 34:1329–1339Google Scholar
  2. 2.
    Zanetti AR, Van Damme P et al (2008) The global impact of vaccination against hepatitis B: a historical overview. Vaccine 26(49):6266–6273Google Scholar
  3. 3.
    Tran TT, Trinh TN, Abe K (2008) New complex recombinant genotype of hepatitis B virus identified in Vietnam. J Virol 82(11):5657–5663CrossRefGoogle Scholar
  4. 4.
    Shen Tao, Gao Jian-mei, Zou Yun-Lian et al (2009) Novel hepatitis B virus subgenotype in the Southern Yunnan Province of China. Intervirology 52:340–346CrossRefGoogle Scholar
  5. 5.
    Van Oven M, Kayser M (2009) Updated comprehensive phylogenetic tree of global human mitochondrial DNA variation. Hum Mutat 30(2):E386–E394CrossRefGoogle Scholar
  6. 6.
    Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucl Acids Symp Ser 41:95–98Google Scholar
  7. 7.
    Thompson JD, Gibson TJ et al (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefGoogle Scholar
  8. 8.
    Tamura K, Peterson D, Peterson N et al (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739CrossRefGoogle Scholar
  9. 9.
    Rozanov M, Plikat U, Chappey C et al (2004) A web-based genotyping resource for viral sequences. Nucleic Acids Res 32:654–659CrossRefGoogle Scholar
  10. 10.
    Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783–779CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Department of Computer ScienceKunming University of Science and Technology KunmingChina
  2. 2.Institute of Biomedical EngineeringKunming University of Science and TechnologyKunmingChina

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