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Comparison of Beta Variable Gene Usage of T Cell Receptor in Peripheral Blood and Synovial Fluid of Rheumatoid Arthritis Patients

  • Jianwei Zhou
  • Cui Kong
  • Xiukui Wang
  • Zhaocai Zhang
  • Chengqiang Jin
  • Qin Song
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)

Abstract

Objective To compare the beta variable (Vβ) gene usage of T cell receptor (TCR) in peripheral blood (PB) and synovial fluid (SF) of the patients with rheumatoid arthritis (RA). Methods The total RNAs were extracted from PB and SF of 12 RA patients and reverse-transcribed and amplified by polymerase chain reaction (PCR), then the TCR Vβ gene usages were detected with real-time fluorescence quantitative PCR with DNA melting curving technique. Results For the four patients with acute RA, Vβ1 and Vβ13.1 were the key advantage usage genes in PB, while in SF that were Vβ10 and Vβ13.1. Except for Vβ11, there were no other genes found to be restrictively used both in PB and SF. For the eight patients with chronic RA, in PB, the main advantage usage genes were Vβ1, Vβ5.1, Vβ7 and Vβ13.1, the limited usage genes were Vβ2, Vβ11, Vβ21 and Vβ22. In SF, the predominant usage genes were Vβ1, Vβ6, Vβ7, Vβ13.1 and Vβ18, the limited usage genes were Vβ2, Vβ10, Vβ11, Vβ12, Vβ17, Vβ22, Vβ23 and Vβ24. Conclusions TCR Vβ gene usages in PB are similar to that in SF of RA patients, and this is probably an indication for the further studies of the pathogenesis and therapy of RA in future.

Keywords

Rheumatoid arthritis T cell receptor Variable beta gene Peripheral blood Synovial fluid Advantage usage Limited usage 

Notes

Acknowledgments

The article is supported by the Provincial Science and Technology Development Project (Grant 2012YD18054), the Provincial Nature Science Foundation (Grant ZR2012HL29), the High School Science and Technology Plan Project (Grant J11LF18), the Population and Family Planning Commission (Grant [2011]13), and the Development Plan Project of Jining Science and Technology Bureau of Shandong Province (Grant [2011]57), the Youth Foundation of Jining Medical College (Grant [2011]).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Jianwei Zhou
    • 1
  • Cui Kong
    • 2
  • Xiukui Wang
    • 3
  • Zhaocai Zhang
    • 1
  • Chengqiang Jin
    • 1
  • Qin Song
    • 4
  1. 1.Clinic LaboratoryThe Affiliated Hospital of Jining Medical CollegeJiningChina
  2. 2.Department of Cardiovascular DiseaseThe Affiliated Hospital of Jining Medical CollegeJiningChina
  3. 3.Department of StomatologyThe Affiliated Hospital of Jining Medical CollegeJiningChina
  4. 4.Department of Immune Rheumatic DiseaseThe Affiliated Hospital of Jining Medical CollegeJiningChina

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