A Fast and Simple HPLC–UV Method for Simultaneous Determination of Emodin and Quinalizarin from Fermentation Broth of Aspergillus. ochraceus lp_0429
A rapid, sensitive and reproducible high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous quantification of Emodin and Quinalizarin from Fermentation Broth of Aspergillus. ochraceus lp_0429. Isocratic RP-HPLC system with C18 column (4.6 mm × 250 mm i.d., 5 μ particle size) and a detector UV-VWD was employed. The mobile phase of 0.05 M disodium hydrogen phosphate and acetonitrile (25:75, v/v) containing 0.5 mL/L triethylamine at pH 5.8 was delivered at flow rate of 0.1 mL/min at ambient temperature. The UV detection of emodin and quinalizarin was set at 254 nm. Total run time was 10 min and retention times for Emodin and Quinalizarin were 2.08 and 6.12 min, respectively.
KeywordsHPLC–UV Emodin Quinalizarin Aspergillus Ochraceus
The financial support of Tianjin Natural Science Foundation (A Study of Biosynthesis Pathway and Induced Mechanism from Reactive Oxygen Species of Emodin by Fermentation from Aspergillus ochraceus, No: 12JCYBJC19000), is gratefully Acknowledged.
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