An Optimal In Vitro Model for Evaluating Anaphylactoid Mediator Release Induced by Herbal Medicine Injection

  • Zheng Xiang
  • Chen-Feng Ji
  • De-Qiang Dou
  • Hang Xiao
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)


The Xue-Sai-Tong injection, a traditional Chinese medicine injection with total saponins extracted from Sanqi Ginseng, was used to treat coronary artery disease. Adverse drug reaction of the injection occurred frequently in recent years. This study was to establish an in vitro model for assaying the mediator release from the degranulation of mast cell and RBL-2H3 cells stimulated by herbal medicine injection. Mediators released from mast cells and RBL-2H3 cells caused by Xue-Sai-Tong injections were assayed by the method of fluorospectrophotometry, ELISA and spectrophotometry respectively. It revealed that histamine release induced by XST injection could not be assayed accurately by the method of fluorospectrophotometry owing to the existence of saponin and unknown components in the injection. Rat peritoneal mast cells was also not an optimal cell model for determining histamine and β-hexosaminidase release due to the higher spontaneous release rate of allergic mediator. So, ELISA could be the suitable method to evaluate the histamine release from RBL-2H3 cells, and this method could also be utilized in evaluating degranulation of herbal medicine injection.


RBL-2H3 Degranulation Saponin Herbal medicine injection 


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Zheng Xiang
    • 1
    • 2
  • Chen-Feng Ji
    • 1
    • 2
  • De-Qiang Dou
    • 1
    • 2
    • 3
  • Hang Xiao
    • 3
  1. 1.Engineering Research Center of Natural Anticancer DrugsMinistry of Education, Harbin University of CommerceHarbinChina
  2. 2.Center of Research on Life Science and Environmental ScienceHarbin University of CommerceHarbinChina
  3. 3.College of PharmacyLiaoning University of Traditional Chinese MedicineDalianChina

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