Nitric Oxide Donor Regulated mRNA Expressions of LTC4 Synthesis Enzymes in Hepatic Ischemia Reperfusion Injury Rats
Cysteinyl LTs were associated with hepatic ischemia and reperfusion (I/R) injury. LTC4 synthesis enzymes including leukotriene C4 synthase (LTC4S), microsomal glutathione S-transferase (mGST) 2 and mGST3 can all conjugate LTA4 and reduced glutathione (GSH) to form LTC4, which involved hepatic ischemia–reperfusion (I/R) injury. This experiment was designed to further investigate the effects of V-PYRRO/NO (a selective liver nitric oxide donor)on the gene expressions of LTC4 synthesis enzymes during hepatic I/R. Adult male SD rats were divided into 3 groups: sham group (Control), I/R group and V-PYRRO/NO + I/R groups. Liver subjected to 1 h of partial hepatic ischemia followed by 5 h of reperfusion, saline or V-PYRRO/NO (1.06 μmol/kg/h) was administered intravenously through all the experimental periods. The mRNA levels of LTC4 synthesis enzymes in rat liver tissue were examined by RT-PCR. We observed that hepatic mRNA expressions of LTC4S and mGST3 were lower whereas mGST2 mRNA levels were higher in V-PYRRO/NO +I/R group than those in I/R group. Compared with control, only mGST3 mRNA was significantly declined in V-PYRRO/NO +I/R groups. These results suggest that NO donor V-PYRRO/NO can downregulate the mRNA expressions of LTC4S and mGST3 but upregulate mGST2 mRNA expressions during hepatic I/R injury.
KeywordsNO donor Microsomal glutathione S-transferase Ischemia reperfusion injury Liver
This work was supported by National Natural Science Foundation of China (No. 81260504) and Educational Commission of Jiangxi Province of China (No. GJJ12073) and Health Department of Jiangxi Province of China (No. 20123175, 2012A131 and 20132018).
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