The Effect of T-2 Toxin on the Apoptosis of Ameloblasts in Rat’s Incisor
Trichothecene mycotoxin T-2 toxin is a common contaminant of food and feed and is also present in processed cereal derived products. Cytotoxic effects of T-2 toxin have been already well described with apoptosis being a major action mechanism. It has been reported that T-2 toxin induced the appearance changes in tooth organ. However, effects on the ameloblasts were until now only reported rarely. 60 SD rats were randomly divided into three groups and received gavages containing T-2 toxin in a dosage of 0, 100, and 200 ng/g b.w., respectively. After 4 weeks treatment, the TdT-mediated dUTP nick end labeling (TUNEL) assays were used to investigate the effect of T-2 toxin on the apoptosis of ameloblasts. The imagination analysis of TUNEL showed that there were significantly more apoptotic ameloblasts in the experiment groups than the control group (P < 0.01). This effect was more obvious in high-dose T-2 toxin administrated group. Thus, T-2 toxin can accelerate apoptosis of ameloblasts in rat’s incisor. This model might provide a potential research tool for studying the influence of T-2 toxin on tooth, and further reveal apoptotic disorders in the tooth development deformity.
KeywordsT-2 toxin Rat ameloblast Apoptosis
This research was supported through funding from Scientific Research Program funded by Shaanxi Provincial Education Department (Program No. 12JK0763).
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